Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI30070
Trapped Gene
Gse1 (ENSMUSG00000031822)
Vector Insertion
Chr 8: 123059855 - 123061328
Public Clones D128H01 (ggtc) D034A06 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000710445 (Chr8:123059757..123059854 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AACTAACGCGTGGACCAAAG Chr8:123059792..123059811 60.17 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000710445 (Chr8:123059757..123059854 +)
Downstram Exon
ENSMUSE00000720605 (Chr8:123061329..123061462 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AACTAACGCGTGGACCAAAG Chr8:123059792..123059811 60.17 50 CGGGTTTGTCTGAACTGCTT Chr8:123061405..123061424 60.29 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000605789 Chr8:123012766..123013051 CTTGGACGCTGGTTTTGTTT Chr8:123012910..123012929 60.15 45
upstream ENSMUSE00000710445 Chr8:123059757..123059854 AACTAACGCGTGGACCAAAG Chr8:123059792..123059811 60.17 50

*** Putative Vector Insertion (Chr 8: 123059855 - 123061328) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000720605 Chr8:123061329..123061462 CGGGTTTGTCTGAACTGCTT Chr8:123061405..123061424 60.29 50
downstream ENSMUSE00000579735 Chr8:123077484..123077702 CATCCCTATGGAAGGGGACT Chr8:123077524..123077543 60.15 55
downstream ENSMUSE00000710125 Chr8:123077484..123077702 CATCCCTATGGAAGGGGACT Chr8:123077524..123077543 60.15 55
downstream ENSMUSE00000213352 Chr8:123086526..123086725 CCAGACTCCATTGACGGTTT Chr8:123086710..123086729 59.97 50
downstream ENSMUSE00000213358 Chr8:123090268..123090440 AAGCGGGAGTCCTGTACAAC Chr8:123090431..123090450 59.21 55
downstream ENSMUSE00000303569 Chr8:123090827..123091024 GCAAGGAGGACATGCGTAAG Chr8:123090939..123090958 60.8 55
downstream ENSMUSE00000213364 Chr8:123091663..123091854 ATGGGGTAGAACGGAGACCT Chr8:123091716..123091735 59.82 55
downstream ENSMUSE00000371311 Chr8:123092061..123092383 AGGAAGGTGGTGGGCTCTAA Chr8:123092300..123092319 61.01 55
downstream ENSMUSE00000303504 Chr8:123092952..123093276 AGCCACTTCTCCTCCTCGTT Chr8:123093135..123093154 60.39 55
downstream ENSMUSE00000303484 Chr8:123094617..123095239 CTGAGGCTCCAGACTTCCTG Chr8:123094977..123094996 60.13 60
downstream ENSMUSE00000213370 Chr8:123096204..123096316 TCTCGTCGTACGAGTCATCG Chr8:123096242..123096261 60.01 55
downstream ENSMUSE00000213351 Chr8:123096536..123096806 TTCCTCTTCTCCGCACTGAT Chr8:123096807..123096826 59.95 50
downstream ENSMUSE00000213366 Chr8:123098071..123098178 GGACAGTGCTCTCTGCTTCA Chr8:123098126..123098145 59.29 55
downstream ENSMUSE00000213350 Chr8:123098834..123099205 CGTGGAATTGATGTGCAAAC Chr8:123099163..123099182 59.97 45
downstream ENSMUSE00000213354 Chr8:123100176..123100454 CTCGCTGTCCTCCTCAGAGT Chr8:123100378..123100397 59.73 60
downstream ENSMUSE00000213357 Chr8:123101871..123101974 GGCTGAGGCTGTAGTTCTGC Chr8:123101945..123101964 60.16 60
downstream ENSMUSE00000351895 Chr8:123102369..123105276 AAGGGGGCTACTGTGGTCTT Chr8:123104382..123104401 59.99 55
downstream ENSMUSE00000712712 Chr8:123102369..123103226 AGAGGAAATAACGCCGACCT Chr8:123102740..123102759 60.1 50
downstream ENSMUSE00000715807 Chr8:123102369..123103380 AACCCCCAAACAGGAAAAAC Chr8:123103316..123103335 60.07 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTTTAATCGCCTTGCAGCAC Chr8:123059903..123059923 60.78 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCGTTCGTGACTGGGAAAAC Chr8:123059901..123059921 61.08 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031822