Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI3041
Trapped Gene
2410012H22Rik (ENSMUSG00000014243)
Vector Insertion
Chr 11: 62090119 - 62094878
Public Clones DC0628 (sanger) IST12774B12 (tigm) IST10891E7 (tigm)
Private Clones OST373852 (lexicon)
Severity of mutation (?) Insertion after 17% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000105758 (Chr11:62094759..62094877 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000105758 (Chr11:62094759..62094877 -)
Downstram Exon
ENSMUSE00000677732 (Chr11:62090120..62090141 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000105758 Chr11:62094759..62094877 No primer for this exon
upstream ENSMUSE00000677732 Chr11:62090120..62090141 No primer for this exon

*** Putative Vector Insertion (Chr 11: 62090119 - 62094878) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000105748 Chr11:62087214..62087316 No primer for this exon
downstream ENSMUSE00000105745 Chr11:62082206..62082310 No primer for this exon
downstream ENSMUSE00000105755 Chr11:62080732..62081108 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTTCTGAGCATCCCAGTTAG Chr11:62094898..62094919 59.09 52.38 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AAGTGTGTGTCTGACCTTTCCA Chr11:62094876..62094898 59.66 45.46 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 AAGAGCTCCTGAGCGAGATG Chr11:62094785..62094805 59.85 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 AAGAGCTCCTGAGCGAGATG Chr11:62094785..62094805 59.85 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000014243