Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI30821
Trapped Gene
1700061G19Rik (ENSMUSG00000024209)
Vector Insertion
Chr 17: 57023284 - 57024173
Public Clones CMHD-GT_534E1-3 (cmhd) IST13773G12 (tigm) IST11182B9 (tigm) IST10169H6 (tigm)
IST10163H10 (tigm) IST14839D11 (tigm) IST10163H10 (tigm) IST14889B4 (tigm)
IST15091G10 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 67% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000283755 (Chr17:57023050..57023283 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GTTGTACGGCTTGTCGGAGT Chr17:57023215..57023234 60.18 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000283755 (Chr17:57023050..57023283 +)
Downstram Exon
ENSMUSE00000139749 (Chr17:57024174..57024391 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GTTGTACGGCTTGTCGGAGT Chr17:57023215..57023234 60.18 55 CCACCTTCCTCTCTGTGCTC Chr17:57024313..57024332 59.99 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000284015 Chr17:57014906..57015103 TGATAAAGTGGCAGCCTGTG Chr17:57015009..57015028 59.86 50
upstream ENSMUSE00000283990 Chr17:57015511..57015819 CTGTGGACACCTGGTCCTCT Chr17:57015611..57015630 60.15 60
upstream ENSMUSE00000283969 Chr17:57016786..57017012 GCAACTGCTCACCTACATCG Chr17:57016949..57016968 59.47 55
upstream ENSMUSE00000376498 Chr17:57019874..57019962 GTCTGGAACGCTTTCATGGT Chr17:57019878..57019897 60.12 50
upstream ENSMUSE00000283885 Chr17:57020369..57020489 TCGCTGAGACCTCAGAAATG Chr17:57020422..57020441 59.11 50
upstream ENSMUSE00000493939 Chr17:57021590..57021670 TCCAGGGCTACTTGAAGCAT Chr17:57021591..57021610 59.84 50
upstream ENSMUSE00000283830 Chr17:57021964..57022113 ACTGAAGCCCAATCAGTGCT Chr17:57022029..57022048 59.87 50
upstream ENSMUSE00000139757 Chr17:57022203..57022409 GCTACCTGCCACTTTGCTTC Chr17:57022279..57022298 60.02 55
upstream ENSMUSE00000139751 Chr17:57022739..57022920 TCCACACCAACAAGAAGCTG Chr17:57022893..57022912 59.87 50
upstream ENSMUSE00000283755 Chr17:57023050..57023283 GTTGTACGGCTTGTCGGAGT Chr17:57023215..57023234 60.18 55

*** Putative Vector Insertion (Chr 17: 57023284 - 57024173) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000139749 Chr17:57024174..57024391 CCACCTTCCTCTCTGTGCTC Chr17:57024313..57024332 59.99 60
downstream ENSMUSE00000139761 Chr17:57024507..57024646 GGGATACGGGTTGACCATTT Chr17:57024553..57024572 60.81 50
downstream ENSMUSE00000283440 Chr17:57025864..57026110 AGGTTACTCCGAGCCTCTCC Chr17:57025907..57025926 59.84 60
downstream ENSMUSE00000693744 Chr17:57028060..57028327 GGCGCTTGAACGAAGATTAG Chr17:57028182..57028201 59.98 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGCACGGGACTACACACAGT Chr17:57023235..57023255 60.23 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGCACGGGACTACACACAGT Chr17:57023235..57023255 60.23 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024209