Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI30826
Trapped Gene
Mapt (ENSMUSG00000018411)
Vector Insertion
Chr 11: 104148524 - 104151221
Public Clones (ggtc) (ggtc) IST11684G12 (tigm) IST14249G3 (tigm) IST12241F10 (tigm)
IST12337E10 (tigm) IST12783G11 (tigm) IST12649D12 (tigm) IST10031E4 (tigm)
IST14136B10 (tigm) IST12241F10 (tigm) IST12444D4 (tigm) IST11131D9 (tigm)
IST10512E6 (tigm) IST11348B3 (tigm) IST11348B3 (tigm) IST13644G12 (tigm)
IST13528F11 (tigm) IST13751H10 (tigm) IST14136B10 (tigm) IST13174F1 (tigm)
IST11995F7 (tigm) IST14388F6 (tigm) IST11435A3 (tigm) IST10021A8 (tigm)
IST14249G3 (tigm) IST10019C3 (tigm) IST12037H10 (tigm) IST12649D12 (tigm)
IST14533D12 (tigm) IST10202F8 (tigm) IST10436C6 (tigm) IST10806G8 (tigm)
IST12037H10 (tigm) IST10131E7 (tigm) IST10054G10 (tigm) IST12376D12 (tigm)
IST12337E10 (tigm) IST10785E6 (tigm) IST12465G2 (tigm) IST11609A12 (tigm)
IST10131E7 (tigm) IST15074B6 (tigm) IST12376D12 (tigm) IST11746H3 (tigm)
IST13751H10 (tigm) IST14388F6 (tigm) IST12465G2 (tigm) IST13644G12 (tigm)
IST10591E5 (tigm) IST10512E6 (tigm) IST10591E5 (tigm) IST11995F7 (tigm)
IST10785E6 (tigm) IST11887E9 (tigm) IST13043H7 (tigm) IST10652E4 (tigm)
IST12227C1 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 9% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000107966 (Chr11:104148437..104148523 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000107966 (Chr11:104148437..104148523 +)
Downstram Exon
ENSMUSE00000107958 (Chr11:104151222..104151308 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000503576 Chr11:104092812..104092921 No primer for this exon
upstream ENSMUSE00000357735 Chr11:104143682..104143794 No primer for this exon
upstream ENSMUSE00000671767 Chr11:104143695..104143794 No primer for this exon
upstream ENSMUSE00000107966 Chr11:104148437..104148523 No primer for this exon

*** Putative Vector Insertion (Chr 11: 104148524 - 104151221) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000107958 Chr11:104151222..104151308 No primer for this exon
downstream ENSMUSE00000710380 Chr11:104156173..104156238 No primer for this exon
downstream ENSMUSE00000721123 Chr11:104156173..104156238 No primer for this exon
downstream ENSMUSE00000671771 Chr11:104159812..104160570 No primer for this exon
downstream ENSMUSE00000107959 Chr11:104159860..104160570 No primer for this exon
downstream ENSMUSE00000107970 Chr11:104163675..104163724 No primer for this exon
downstream ENSMUSE00000671756 Chr11:104163675..104163724 No primer for this exon
downstream ENSMUSE00000107957 Chr11:104166526..104166723 No primer for this exon
downstream ENSMUSE00000671755 Chr11:104167940..104168072 No primer for this exon
downstream ENSMUSE00000708822 Chr11:104167940..104168072 No primer for this exon
downstream ENSMUSE00000713313 Chr11:104167940..104168072 No primer for this exon
downstream ENSMUSE00000671768 Chr11:104169195..104169248 No primer for this exon
downstream ENSMUSE00000107971 Chr11:104171583..104171848 No primer for this exon
downstream ENSMUSE00000671764 Chr11:104171583..104171848 No primer for this exon
downstream ENSMUSE00000107965 Chr11:104179471..104179563 No primer for this exon
downstream ENSMUSE00000671763 Chr11:104179471..104179563 No primer for this exon
downstream ENSMUSE00000107964 Chr11:104182665..104182746 No primer for this exon
downstream ENSMUSE00000671762 Chr11:104182665..104182746 No primer for this exon
downstream ENSMUSE00000107967 Chr11:104183742..104183854 No primer for this exon
downstream ENSMUSE00000671761 Chr11:104183742..104183854 No primer for this exon
downstream ENSMUSE00000381052 Chr11:104189286..104189555 No primer for this exon
downstream ENSMUSE00000509438 Chr11:104189286..104189555 No primer for this exon
downstream ENSMUSE00000671766 Chr11:104189286..104189501 No primer for this exon
downstream ENSMUSE00000671774 Chr11:104189286..104189493 No primer for this exon
downstream ENSMUSE00000671772 Chr11:104190416..104190492 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTCCCACCACTCCATTCATT Chr11:104148539..104148559 59.78 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTCCCACCACTCCATTCATT Chr11:104148539..104148559 59.78 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018411