Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI30903
Trapped Gene
Gcnt2 (ENSMUSG00000021360)
Vector Insertion
Chr 13: 40984580 - 41013002
Public Clones (ggtc) (ggtc) (cmhd) IST12773B11 (tigm) IST12324A7 (tigm) IST12324A7 (tigm)
IST12326B6 (tigm) IST14812C9 (tigm) IST12326B6 (tigm) IST12773B11 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000683057 (Chr13:40984564..40984579 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000683057 (Chr13:40984564..40984579 +)
Downstram Exon
ENSMUSE00000288319 (Chr13:41013003..41014170 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000683059 Chr13:40955501..40956642 No primer for this exon
upstream ENSMUSE00000500581 Chr13:40982535..40983654 No primer for this exon
upstream ENSMUSE00000683058 Chr13:40982736..40983658 No primer for this exon
upstream ENSMUSE00000683057 Chr13:40984564..40984579 No primer for this exon

*** Putative Vector Insertion (Chr 13: 40984580 - 41013002) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000288319 Chr13:41013003..41014170 No primer for this exon
downstream ENSMUSE00000446426 Chr13:41048945..41049040 No primer for this exon
downstream ENSMUSE00000446420 Chr13:41053521..41056260 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AAAGCGCCTGAGCTGCTAAT Chr13:40999615..40999635 61.52 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTGGCCAGACATACTGACG Chr13:40999613..40999633 58.75 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000021360