Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI30976
Trapped Gene
D130003B22Rik (ENSMUSG00000024459)
Vector Insertion
Chr 17: 37121005 - 37124469
Public Clones 5SE086G01 (ggtc) IST11006A12 (tigm) IST10385A6 (tigm) IST14949H5 (tigm)
IST12536C5 (tigm) IST12355A8 (tigm) IST10049E1 (tigm) IST11566B5 (tigm)
IST11975H2 (tigm) IST12315D12 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 98% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000696604 (Chr17:37124361..37124468 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AATCACTGTTGGCCTGCTTC Chr17:37124418..37124437 60.26 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000696604 (Chr17:37124361..37124468 -)
Downstram Exon
ENSMUSE00000696602 (Chr17:37121006..37122047 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AATCACTGTTGGCCTGCTTC Chr17:37124418..37124437 60.26 50 GAACAAGCATGTGGGAGGTT Chr17:37121169..37121188 59.97 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000696612 Chr17:37126374..37126449 CTGCGCTCTCTACCCTCCTA Chr17:37126420..37126439 59.73 60
upstream ENSMUSE00000696611 Chr17:37125880..37126149 TTCGTGGACGACACACAGTT Chr17:37126053..37126072 60.2 50
upstream ENSMUSE00000696609 Chr17:37125450..37125725 CTCCGCACATACCTGGAGAT Chr17:37125476..37125495 60.1 55
upstream ENSMUSE00000696606 Chr17:37124600..37124875 GGCATCTGTGGTGGTACCTT Chr17:37124672..37124691 59.85 55
upstream ENSMUSE00000696604 Chr17:37124361..37124468 AATCACTGTTGGCCTGCTTC Chr17:37124418..37124437 60.26 50
upstream ENSMUSE00000696602 Chr17:37121006..37122047 AACCTCCCACATGCTTGTTC Chr17:37121191..37121210 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACCTGCCAAATTCTCACCAT Chr17:37121483..37121503 59.41 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACCTGCCAAATTCTCACCAT Chr17:37121483..37121503 59.41 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024459