Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31027
Trapped Gene
Zfp97 (ENSMUSG00000067034)
Vector Insertion
Chr 17: 17214156 - 17221710
Public Clones (sanger) (sanger) (sanger) (sanger) (sanger) 3SD142A11 (ggtc)
IST10086F8 (tigm) IST14668B9 (tigm) IST14644H4 (tigm) IST14602C7 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000703501 (Chr17:17214126..17214155 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000703501 (Chr17:17214126..17214155 +)
Downstram Exon
ENSMUSE00000703496 (Chr17:17221711..17221730 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000703501 Chr17:17214126..17214155 No primer for this exon

*** Putative Vector Insertion (Chr 17: 17214156 - 17221710) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000703488 Chr17:17221711..17221730 No primer for this exon
downstream ENSMUSE00000703496 Chr17:17221711..17221730 No primer for this exon
downstream ENSMUSE00000703485 Chr17:17221953..17221959 No primer for this exon
downstream ENSMUSE00000703493 Chr17:17221953..17221959 No primer for this exon
downstream ENSMUSE00000578014 Chr17:17222485..17222611 CAAAGCCCATTCTTCCTGAG Chr17:17222544..17222563 59.81 50
downstream ENSMUSE00000658216 Chr17:17222485..17222611 CAAAGCCCATTCTTCCTGAG Chr17:17222544..17222563 59.81 50
downstream ENSMUSE00000613608 Chr17:17222854..17222914 TGGGTCTTCCAGAATTTTCA Chr17:17222913..17222932 58.12 40
downstream ENSMUSE00000577995 Chr17:17224208..17226592 CACTGCATTATCCGGGTTTT Chr17:17226481..17226500 59.82 45
downstream ENSMUSE00000658214 Chr17:17224208..17225028 TTTCTTGCAAAGGCTCCATC Chr17:17224291..17224310 60.33 45
downstream ENSMUSE00000658212 Chr17:17226206..17226593 CACTGCATTATCCGGGTTTT Chr17:17226481..17226500 59.82 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTAATCGCCTTGCAGCACAT Chr17:17214206..17214226 61.2 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCATTAGACCTTTCTGTTTGTAGG Chr17:17214136..17214160 58.47 41.67 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000067034