Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31057
Trapped Gene
Srgn (ENSMUSG00000020077)
Vector Insertion
Chr 10: 61970660 - 61990200
Public Clones IST10313G5 (tigm) IST10313F8 (tigm) IST12521A4 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000666257 (Chr10:61990087..61990199 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000666257 (Chr10:61990087..61990199 -)
Downstram Exon
ENSMUSE00000666254 (Chr10:61970661..61970686 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000666257 Chr10:61990087..61990199 No primer for this exon
upstream ENSMUSE00000666254 Chr10:61970661..61970686 No primer for this exon

*** Putative Vector Insertion (Chr 10: 61970660 - 61990200) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000100394 Chr10:61970390..61970504 No primer for this exon
downstream ENSMUSE00000666253 Chr10:61970390..61970489 No primer for this exon
downstream ENSMUSE00000666256 Chr10:61970390..61970584 No primer for this exon
downstream ENSMUSE00000100395 Chr10:61960527..61960671 No primer for this exon
downstream ENSMUSE00000666252 Chr10:61957432..61957854 No primer for this exon
downstream ENSMUSE00000100393 Chr10:61957177..61957854 No primer for this exon
downstream ENSMUSE00000666255 Chr10:61956581..61957854 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATCTCACCGGAAGGATAATCG Chr10:61972143..61972164 60.29 47.62 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AAGGACGTGACTGGGAAAAC Chr10:61972134..61972154 59.04 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020077