Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31119
Trapped Gene
Cybb (ENSMUSG00000015340)
Vector Insertion
Chr X: 9021673 - 9024073
Public Clones IST14258D10 (tigm) IST12068A6 (tigm) IST14271E9 (tigm) IST14431C6 (tigm)
IST10851G4 (tigm) IST12018E7 (tigm) IST12050E7 (tigm) IST14502D2 (tigm)
IST11690B8 (tigm) IST13235C7 (tigm) IST11089F11 (tigm) IST11731B9 (tigm)
IST10744A5 (tigm) IST12477H2 (tigm) IST14645B7 (tigm) IST10616C4 (tigm)
IST14294E1 (tigm) IST14561B4 (tigm) IST14573C12 (tigm) IST14344E11 (tigm)
IST14708A2 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 67% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000207131 (ChrX:9023819..9024072 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000207131 (ChrX:9023819..9024072 -)
Downstram Exon
ENSMUSE00000207130 (ChrX:9021674..9021836 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000349721 ChrX:9046332..9046417 No primer for this exon
upstream ENSMUSE00000207125 ChrX:9044209..9044304 No primer for this exon
upstream ENSMUSE00000207129 ChrX:9042770..9042880 No primer for this exon
upstream ENSMUSE00000207120 ChrX:9034086..9034170 No primer for this exon
upstream ENSMUSE00000207124 ChrX:9032260..9032405 No primer for this exon
upstream ENSMUSE00000207123 ChrX:9030712..9030902 No primer for this exon
upstream ENSMUSE00000207122 ChrX:9027808..9027937 No primer for this exon
upstream ENSMUSE00000207132 ChrX:9026767..9026859 No primer for this exon
upstream ENSMUSE00000207131 ChrX:9023819..9024072 No primer for this exon
upstream ENSMUSE00000207130 ChrX:9021674..9021836 No primer for this exon

*** Putative Vector Insertion (Chr X: 9021673 - 9024073) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000207127 ChrX:9019659..9019805 No primer for this exon
downstream ENSMUSE00000207128 ChrX:9017021..9017145 No primer for this exon
downstream ENSMUSE00000410012 ChrX:9013154..9015469 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000015340