Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31280
Trapped Gene
Nola2 (ENSMUSG00000001056)
Vector Insertion
Chr 11: 51433480 - 51433583
Public Clones IST12434B12 (tigm) IST10438D4 (tigm) IST10438C4 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 35% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000364720 (Chr11:51433288..51433479 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000364720 (Chr11:51433288..51433479 +)
Downstram Exon
ENSMUSE00000502326 (Chr11:51433584..51433653 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000364720 Chr11:51433288..51433479 No primer for this exon

*** Putative Vector Insertion (Chr 11: 51433480 - 51433583) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000502326 Chr11:51433584..51433653 No primer for this exon
downstream ENSMUSE00000103695 Chr11:51435987..51436092 No primer for this exon
downstream ENSMUSE00000294609 Chr11:51436594..51436931 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTGCATCAAGAAGGGTGAGG Chr11:51433467..51433487 60.66 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTGCATCAAGAAGGGTGAGG Chr11:51433467..51433487 60.66 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001056