Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI31318
Trapped Gene
Amph (
ENSMUSG00000021314
)
Vector Insertion
Chr 13: 19217018 - 19229404
Public Clones
IST11203E4 (tigm)
IST10512H10 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 67% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000116768 (Chr13:19216895..19217017 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000116768 (Chr13:19216895..19217017 +)
Downstram Exon
ENSMUSE00000116763 (Chr13:19229405..19229611 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000323182
Chr13:19040240..19040370
No primer for this exon
upstream
ENSMUSE00000323322
Chr13:19137822..19137902
No primer for this exon
upstream
ENSMUSE00000323317
Chr13:19169200..19169254
No primer for this exon
upstream
ENSMUSE00000323312
Chr13:19176074..19176168
No primer for this exon
upstream
ENSMUSE00000116783
Chr13:19178392..19178487
No primer for this exon
upstream
ENSMUSE00000116774
Chr13:19186622..19186729
No primer for this exon
upstream
ENSMUSE00000116767
Chr13:19187876..19187961
No primer for this exon
upstream
ENSMUSE00000116765
Chr13:19191762..19191837
No primer for this exon
upstream
ENSMUSE00000116779
Chr13:19192441..19192523
No primer for this exon
upstream
ENSMUSE00000116771
Chr13:19194741..19194879
No primer for this exon
upstream
ENSMUSE00000350589
Chr13:19196100..19196228
No primer for this exon
upstream
ENSMUSE00000116777
Chr13:19204972..19205088
No primer for this exon
upstream
ENSMUSE00000116764
Chr13:19208106..19208129
No primer for this exon
upstream
ENSMUSE00000116773
Chr13:19210021..19210044
No primer for this exon
upstream
ENSMUSE00000116782
Chr13:19212465..19212497
No primer for this exon
upstream
ENSMUSE00000344330
Chr13:19213144..19213188
No primer for this exon
upstream
ENSMUSE00000116768
Chr13:19216895..19217017
No primer for this exon
*** Putative Vector Insertion (Chr 13: 19217018 - 19229404) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000116763
Chr13:19229405..19229611
No primer for this exon
downstream
ENSMUSE00000116766
Chr13:19231041..19231301
No primer for this exon
downstream
ENSMUSE00000116769
Chr13:19233834..19233935
No primer for this exon
downstream
ENSMUSE00000338773
Chr13:19241605..19242782
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TATTAATCGCCTTGCAGCAC
Chr13:19220066..19220086
58.94
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GAGGTATCGTGACTGGGAAAA
Chr13:19220062..19220083
59.04
47.62
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000021314
