Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI31368
Trapped Gene
Wnt3a (
ENSMUSG00000009900
)
Vector Insertion
Chr 11: 59088641 - 59104255
Public Clones
IST11531E1 (tigm)
IST14765C8 (tigm)
IST11138F8 (tigm)
IST14765C8 (tigm)
IST12504H5 (tigm)
IST12504H5 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 7% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000397954 (Chr11:59104079..59104254 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000397954 (Chr11:59104079..59104254 -)
Downstram Exon
ENSMUSE00000104673 (Chr11:59088642..59088883 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000397954
Chr11:59104079..59104254
No primer for this exon
upstream
ENSMUSE00000104673
Chr11:59088642..59088883
No primer for this exon
*** Putative Vector Insertion (Chr 11: 59088641 - 59104255) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000104669
Chr11:59069782..59070047
No primer for this exon
downstream
ENSMUSE00000370057
Chr11:59061556..59063611
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GAATCAGGTAATCGCCTTGC
Chr11:59101192..59101212
59.67
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GAGAGCAATGGCTCAAGAGG
Chr11:59098266..59098286
60.1
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000009900
