Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31368
Trapped Gene
Wnt3a (ENSMUSG00000009900)
Vector Insertion
Chr 11: 59088641 - 59104255
Public Clones IST11138F8 (tigm) IST14765C8 (tigm) IST12504H5 (tigm) IST12504H5 (tigm)
IST11531E1 (tigm) IST14765C8 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 7% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000397954 (Chr11:59104079..59104254 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000397954 (Chr11:59104079..59104254 -)
Downstram Exon
ENSMUSE00000104673 (Chr11:59088642..59088883 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000397954 Chr11:59104079..59104254 No primer for this exon
upstream ENSMUSE00000104673 Chr11:59088642..59088883 No primer for this exon

*** Putative Vector Insertion (Chr 11: 59088641 - 59104255) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000104669 Chr11:59069782..59070047 No primer for this exon
downstream ENSMUSE00000370057 Chr11:59061556..59063611 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAATCAGGTAATCGCCTTGC Chr11:59101192..59101212 59.67 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGAGCAATGGCTCAAGAGG Chr11:59098266..59098286 60.1 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000009900