Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31415
Trapped Gene
Insr (ENSMUSG00000005534)
Vector Insertion
Chr 8: 3174613 - 3185153
Public Clones IST14867C6 (tigm) IST13486D10 (tigm) IST14867C6 (tigm) IST14508H10 (tigm)
IST14203A9 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 54% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000611282 (Chr8:3184951..3185152 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000611282 (Chr8:3184951..3185152 -)
Downstram Exon
ENSMUSE00000233977 (Chr8:3174614..3174888 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000638456 Chr8:3279029..3279128 No primer for this exon
upstream ENSMUSE00000611295 Chr8:3258383..3258934 No primer for this exon
upstream ENSMUSE00000611294 Chr8:3211379..3211700 No primer for this exon
upstream ENSMUSE00000611293 Chr8:3204630..3204778 No primer for this exon
upstream ENSMUSE00000611267 Chr8:3202890..3203034 No primer for this exon
upstream ENSMUSE00000638453 Chr8:3198061..3198275 No primer for this exon
upstream ENSMUSE00000611287 Chr8:3194795..3194921 No primer for this exon
upstream ENSMUSE00000611286 Chr8:3192546..3192802 No primer for this exon
upstream ENSMUSE00000611285 Chr8:3189125..3189292 No primer for this exon
upstream ENSMUSE00000611282 Chr8:3184951..3185152 No primer for this exon
upstream ENSMUSE00000233977 Chr8:3174614..3174888 No primer for this exon

*** Putative Vector Insertion (Chr 8: 3174613 - 3185153) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000233970 Chr8:3173480..3173619 No primer for this exon
downstream ENSMUSE00000611280 Chr8:3169709..3169868 No primer for this exon
downstream ENSMUSE00000611279 Chr8:3167502..3167604 No primer for this exon
downstream ENSMUSE00000611278 Chr8:3165518..3165585 No primer for this exon
downstream ENSMUSE00000611277 Chr8:3163237..3163481 No primer for this exon
downstream ENSMUSE00000611276 Chr8:3161681..3161791 No primer for this exon
downstream ENSMUSE00000611274 Chr8:3161339..3161498 No primer for this exon
downstream ENSMUSE00000611273 Chr8:3159453..3159582 No primer for this exon
downstream ENSMUSE00000611272 Chr8:3158696..3158830 No primer for this exon
downstream ENSMUSE00000569243 Chr8:3155401..3156023 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC Chr8:3176082..3176102 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTCAGTGACTGTTTCCCACCT Chr8:3176129..3176150 59.61 47.62 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000005534