Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31637
Trapped Gene
Sept1 (ENSMUSG00000000486)
Vector Insertion
Chr 7: 134360416 - 134361257
Public Clones (cmhd) PST19854-NR (escells) IST10381H4 (tigm) IST13069F4 (tigm)
IST13754F9 (tigm) IST10187D11 (tigm) IST12009D11 (tigm) IST10442H6 (tigm)
IST13079F4 (tigm) IST10796A1 (tigm) IST12991E6 (tigm) IST10989A1 (tigm)
IST10756E4 (tigm) IST13079H8 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 28% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000201860 (Chr7:134361133..134361256 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000201860 (Chr7:134361133..134361256 -)
Downstram Exon
ENSMUSE00000669452 (Chr7:134360417..134360549 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000669447 Chr7:134361921..134362012 No primer for this exon
upstream ENSMUSE00000669453 Chr7:134361921..134361967 No primer for this exon
upstream ENSMUSE00000291022 Chr7:134361655..134361745 No primer for this exon
upstream ENSMUSE00000649239 Chr7:134361655..134361817 No primer for this exon
upstream ENSMUSE00000201863 Chr7:134361485..134361571 No primer for this exon
upstream ENSMUSE00000201860 Chr7:134361133..134361256 No primer for this exon
upstream ENSMUSE00000669452 Chr7:134360417..134360549 No primer for this exon

*** Putative Vector Insertion (Chr 7: 134360416 - 134361257) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000588043 Chr7:134360415..134360549 No primer for this exon
downstream ENSMUSE00000632379 Chr7:134360301..134360321 No primer for this exon
downstream ENSMUSE00000201858 Chr7:134360204..134360299 No primer for this exon
downstream ENSMUSE00000669446 Chr7:134360204..134360321 No primer for this exon
downstream ENSMUSE00000201867 Chr7:134359382..134359483 No primer for this exon
downstream ENSMUSE00000201865 Chr7:134359173..134359269 No primer for this exon
downstream ENSMUSE00000201862 Chr7:134358795..134358960 No primer for this exon
downstream ENSMUSE00000201864 Chr7:134358463..134358553 No primer for this exon
downstream ENSMUSE00000632378 Chr7:134358157..134358358 No primer for this exon
downstream ENSMUSE00000669444 Chr7:134357976..134358358 No primer for this exon
downstream ENSMUSE00000669451 Chr7:134357964..134358358 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCCAATTCTCTGTCCCCATA Chr7:134361256..134361276 59.89 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCCAATTCTCTGTCCCCATA Chr7:134361256..134361276 59.89 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000000486