Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31651
Trapped Gene
Lars2 (ENSMUSG00000035202)
Vector Insertion
Chr 9: 123368738 - 123370616
Public Clones (sanger) (sanger) IST13019G10 (tigm) IST14435B8 (tigm) IST14265E12 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 94% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000252264 (Chr9:123368610..123368737 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGACCATTATGCCTGGGATT Chr9:123368638..123368657 60.15 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000252264 (Chr9:123368610..123368737 +)
Downstram Exon
ENSMUSE00000252250 (Chr9:123370617..123371780 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGACCATTATGCCTGGGATT Chr9:123368638..123368657 60.15 45 GGGCCTGTGATGGTAAAAGA Chr9:123371347..123371366 59.93 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000252675 Chr9:123276054..123276225 TGGGTCTTGCTCGTGTAATG Chr9:123276092..123276111 59.72 50
upstream ENSMUSE00000252643 Chr9:123280973..123281221 GTTAGCAGGCCACCTGTCAT Chr9:123281048..123281067 60.14 55
upstream ENSMUSE00000252624 Chr9:123286819..123286947 TGTCTACACCCTCAGCGACA Chr9:123286893..123286912 60.46 55
upstream ENSMUSE00000252606 Chr9:123301901..123301992 AATTTGCACCCTGAAAGCTG Chr9:123301964..123301983 60.25 45
upstream ENSMUSE00000498155 Chr9:123304069..123304129 ACATGAGGAAGCAGCTCGAC Chr9:123304080..123304099 60.56 55
upstream ENSMUSE00000252559 Chr9:123318732..123318821 AAGCAGGACTGGCCTATCAA Chr9:123318796..123318815 59.84 50
upstream ENSMUSE00000252543 Chr9:123320991..123321134 CAGTGCTAGCCAATGAGCAG Chr9:123321022..123321041 59.76 55
upstream ENSMUSE00000252522 Chr9:123327297..123327404 TCCCAGAGTGGTACGGAATC Chr9:123327322..123327341 59.93 55
upstream ENSMUSE00000252498 Chr9:123327753..123327912 AAGCCTTGCAGAAGGCACTA Chr9:123327880..123327899 60.15 50
upstream ENSMUSE00000252474 Chr9:123336565..123336669 AGGAGGTCCCCATCGTTATC Chr9:123336607..123336626 60.15 55
upstream ENSMUSE00000252455 Chr9:123341004..123341119 GGGCCTACCCTACTCTGAGG Chr9:123341050..123341069 60.09 65
upstream ENSMUSE00000252428 Chr9:123345235..123345518 CATCTTTAACGGGCAGAGGA Chr9:123345449..123345468 60.21 50
upstream ENSMUSE00000252414 Chr9:123347253..123347351 GAGAGACGGACACCATGGAC Chr9:123347273..123347292 60.54 60
upstream ENSMUSE00000252399 Chr9:123350582..123350719 TCAGTCATTTTTGCCACGAC Chr9:123350680..123350699 59.7 45
upstream ENSMUSE00000252389 Chr9:123361863..123361963 AGACATTCCGCCTTCCATCT Chr9:123361907..123361926 60.99 50
upstream ENSMUSE00000252367 Chr9:123362329..123362511 AGATTGTGGCCCAATATGGA Chr9:123362422..123362441 60.15 45
upstream ENSMUSE00000252345 Chr9:123363828..123363997 AAGATTCATCGAGGCAAGGA Chr9:123363883..123363902 59.77 45
upstream ENSMUSE00000252314 Chr9:123364078..123364155 TTCTCAGCTGATGGGACTCA Chr9:123364122..123364141 59.5 50
upstream ENSMUSE00000252287 Chr9:123365011..123365122 GCCCCTCATGTAACCTCAGA Chr9:123365092..123365111 60.07 55
upstream ENSMUSE00000252264 Chr9:123368610..123368737 TGACCATTATGCCTGGGATT Chr9:123368638..123368657 60.15 45

*** Putative Vector Insertion (Chr 9: 123368738 - 123370616) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000252250 Chr9:123370617..123371780 GGGCCTGTGATGGTAAAAGA Chr9:123371347..123371366 59.93 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGATGGCTGTTCTGGTGAG Chr9:123368724..123368744 59.42 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAAACCTGACATGGTGCAGA Chr9:123368708..123368728 59.68 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000035202