Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31817
Trapped Gene
Rassf1 (ENSMUSG00000010067)
Vector Insertion
Chr 9: 107454178 - 107456456
Public Clones IST14390H10 (tigm) IST12126G12 (tigm) IST14390H10 (tigm) IST13142H9 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000583263 (Chr9:107453928..107454177 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000583263 (Chr9:107453928..107454177 +)
Downstram Exon
ENSMUSE00000720513 (Chr9:107456457..107456575 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000716703 Chr9:107453898..107454177 No primer for this exon
upstream ENSMUSE00000583263 Chr9:107453928..107454177 No primer for this exon

*** Putative Vector Insertion (Chr 9: 107454178 - 107456456) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000720513 Chr9:107456457..107456575 No primer for this exon
downstream ENSMUSE00000583262 Chr9:107456469..107456575 No primer for this exon
downstream ENSMUSE00000254340 Chr9:107457090..107457256 No primer for this exon
downstream ENSMUSE00000710890 Chr9:107458942..107459082 No primer for this exon
downstream ENSMUSE00000221062 Chr9:107459784..107459888 No primer for this exon
downstream ENSMUSE00000221059 Chr9:107460101..107460398 No primer for this exon
downstream ENSMUSE00000221063 Chr9:107460518..107460633 No primer for this exon
downstream ENSMUSE00000254319 Chr9:107463730..107464591 No primer for this exon
downstream ENSMUSE00000720089 Chr9:107463730..107464594 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AAAGGGAGGCAGCACTAATC Chr9:107454214..107454234 58.41 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGCACCGTGACTGGGAAAAC Chr9:107454224..107454244 62.43 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000010067