Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI31924
Trapped Gene
1110051M20Rik (ENSMUSG00000040591)
Vector Insertion
Chr 2: 91119346 - 91122013
Public Clones IST14764B12 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 78% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000302896 (Chr2:91121903..91122012 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AAAGCATCATGGGATCAACC Chr2:91121912..91121931 59.76 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000302896 (Chr2:91121903..91122012 -)
Downstram Exon
ENSMUSE00000302888 (Chr2:91119347..91119410 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AAAGCATCATGGGATCAACC Chr2:91121912..91121931 59.76 45 GTCGTGCATCAGGTCTCCTT Chr2:91119354..91119373 60.27 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000600112 Chr2:91284726..91284837 TGGAAACTACCCGGTCAAAG Chr2:91284773..91284792 59.96 50
upstream ENSMUSE00000662037 Chr2:91284726..91284798 TGGAAACTACCCGGTCAAAG Chr2:91284773..91284792 59.96 50
upstream ENSMUSE00000687518 Chr2:91284726..91284835 TGGAAACTACCCGGTCAAAG Chr2:91284773..91284792 59.96 50
upstream ENSMUSE00000662036 Chr2:91262023..91262125 CGTACATGGAGGATGCAGTG Chr2:91262088..91262107 60.14 55
upstream ENSMUSE00000302929 Chr2:91223886..91224016 AACAGCGTATGCCAAGGAAC Chr2:91223993..91224012 60.14 50
upstream ENSMUSE00000687517 Chr2:91223886..91224016 AACAGCGTATGCCAAGGAAC Chr2:91223993..91224012 60.14 50
upstream ENSMUSE00000302919 Chr2:91144906..91144990 ACTTCCCGCTGGAGCTTACT Chr2:91144920..91144939 60.4 55
upstream ENSMUSE00000687516 Chr2:91144906..91144990 ACTTCCCGCTGGAGCTTACT Chr2:91144920..91144939 60.4 55
upstream ENSMUSE00000450424 Chr2:91124392..91124477 TTCCTTTTCGCCTTCCAGAT Chr2:91124409..91124428 61.06 45
upstream ENSMUSE00000302903 Chr2:91122622..91122817 TCTATCAGCGCCTGGAAAAC Chr2:91122645..91122664 60.35 50
upstream ENSMUSE00000302896 Chr2:91121903..91122012 AAAGCATCATGGGATCAACC Chr2:91121912..91121931 59.76 45
upstream ENSMUSE00000302888 Chr2:91119347..91119410 AAGGAGACCTGATGCACGAC Chr2:91119376..91119395 60.27 55

*** Putative Vector Insertion (Chr 2: 91119346 - 91122013) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000662035 Chr2:91118402..91119114 GCACATGCTCTCAGGAATCA Chr2:91118529..91118548 59.95 50
downstream ENSMUSE00000600111 Chr2:91118401..91119114 GCACATGCTCTCAGGAATCA Chr2:91118529..91118548 59.95 50
downstream ENSMUSE00000687519 Chr2:91117132..91117291 AAAGCTGAGGGCACTGTCTC Chr2:91117153..91117172 59.6 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000040591