Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI32003
Trapped Gene
Slc6a15 (ENSMUSG00000019894)
Vector Insertion
Chr 10: 102830788 - 102832005
Public Clones IST12249A5 (tigm) IST12249A5 (tigm) IST12590D1 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000573998 (Chr10:102830477..102830787 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000573998 (Chr10:102830477..102830787 +)
Downstram Exon
ENSMUSE00000665626 (Chr10:102832006..102832102 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000573998 Chr10:102830477..102830787 No primer for this exon

*** Putative Vector Insertion (Chr 10: 102830788 - 102832005) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000665626 Chr10:102832006..102832102 No primer for this exon
downstream ENSMUSE00000367350 Chr10:102851661..102851975 No primer for this exon
downstream ENSMUSE00000098893 Chr10:102856025..102856182 No primer for this exon
downstream ENSMUSE00000098884 Chr10:102856535..102856661 No primer for this exon
downstream ENSMUSE00000098887 Chr10:102857624..102857805 No primer for this exon
downstream ENSMUSE00000098886 Chr10:102862816..102862926 No primer for this exon
downstream ENSMUSE00000098885 Chr10:102866919..102867160 No primer for this exon
downstream ENSMUSE00000098883 Chr10:102867261..102867453 No primer for this exon
downstream ENSMUSE00000098890 Chr10:102870837..102871029 No primer for this exon
downstream ENSMUSE00000098862 Chr10:102872287..102872446 No primer for this exon
downstream ENSMUSE00000098876 Chr10:102879267..102879429 No primer for this exon
downstream ENSMUSE00000351421 Chr10:102880657..102882011 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000019894