Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI32252
Trapped Gene
Cwf19l2 (ENSMUSG00000025898)
Vector Insertion
Chr 9: 3460132 - 3475482
Public Clones (sanger) (sanger) (sanger) (sanger) (sanger) IST10618F11 (tigm)
IST13650E8 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000261286 (Chr9:3460051..3460131 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000261286 (Chr9:3460051..3460131 +)
Downstram Exon
ENSMUSE00000261261 (Chr9:3475483..3475584 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon AATGATGTGGGCAAAACCTC Chr9:3475551..3475570 59.8 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000153958 Chr9:3404085..3404190 GCGAAAAGAACAGACCCGTA Chr9:3404145..3404164 60.25 50
upstream ENSMUSE00000153955 Chr9:3409966..3410076 GTAAGCGACTTCGGGATGAG Chr9:3410006..3410025 59.84 55
upstream ENSMUSE00000441274 Chr9:3411329..3411451 GAAAAAGGACAAGCATTCAAAAA Chr9:3411352..3411374 59.65 30.43
upstream ENSMUSE00000540178 Chr9:3417869..3417982 GAAGAATGTGACAGCCACGA Chr9:3417956..3417975 59.84 50
upstream ENSMUSE00000540177 Chr9:3418656..3418775 CAAATAGAGCGGGAGAAAACC Chr9:3418740..3418760 60.08 47.62
upstream ENSMUSE00000441251 Chr9:3421286..3421376 GGACAGGTCTCCCATCAAAA Chr9:3421332..3421351 59.9 50
upstream ENSMUSE00000370457 Chr9:3428668..3428783 GCGGATTAAGCTGGCTAAGA Chr9:3428686..3428705 59.59 50
upstream ENSMUSE00000153948 Chr9:3430438..3431087 TGATCCACCAGGAAGAGGAC Chr9:3431037..3431056 60.05 55
upstream ENSMUSE00000153953 Chr9:3441055..3441145 AAGTTGGGGGCCAAGATTAT Chr9:3441101..3441120 59.67 45
upstream ENSMUSE00000153946 Chr9:3442961..3443047 GCAAAAAGATTGGGCGTAGA Chr9:3443027..3443046 60.21 45
upstream ENSMUSE00000153949 Chr9:3447237..3447353 TGAGCAGCCCTAGAGAAACC Chr9:3447298..3447317 59.57 55
upstream ENSMUSE00000153950 Chr9:3450014..3450151 AAGGGTCCGATACTTTCCTGA Chr9:3450037..3450057 59.95 47.62
upstream ENSMUSE00000153952 Chr9:3454540..3454752 ATTGTTGCAATCGGTGTGAA Chr9:3454732..3454751 59.97 40
upstream ENSMUSE00000261328 Chr9:3456733..3456849 CATCATCAAGCAGCCACTGT Chr9:3456799..3456818 59.86 50
upstream ENSMUSE00000261307 Chr9:3458734..3458889 CCCAAAGGAAGTGGGTGATA Chr9:3458850..3458869 59.78 50
upstream ENSMUSE00000261286 Chr9:3460051..3460131 No primer for this exon

*** Putative Vector Insertion (Chr 9: 3460132 - 3475482) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000261261 Chr9:3475483..3475584 AATGATGTGGGCAAAACCTC Chr9:3475551..3475570 59.8 45
downstream ENSMUSE00000407798 Chr9:3477817..3479236 GTCACCCAAACCATGTTTCC Chr9:3478149..3478168 60.07 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAATCGCCTTGCAGCACAT Chr9:3466182..3466202 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAAAACGTGACTGGGAAAACC Chr9:3472178..3472199 60.75 47.62 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000025898