Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI32373
Trapped Gene
Adam32 (ENSMUSG00000037437)
Vector Insertion
Chr 8: 26031759 - 26032808
Public Clones IST12589A4 (tigm) IST12522A9 (tigm) IST12410A12 (tigm) IST12000A8 (tigm)
IST12622C3 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 14% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000408003 (Chr8:26032732..26032807 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000408003 (Chr8:26032732..26032807 -)
Downstram Exon
ENSMUSE00000353634 (Chr8:26031760..26031836 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon CTGAGTTCGGGTAGCCTTTG Chr8:26031769..26031788 59.87 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000511384 Chr8:26059192..26059273 ATGCTGCACACACTGCTGCT Chr8:26059236..26059255 63.26 55
upstream ENSMUSE00000714616 Chr8:26059192..26059267 ATGCTGCACACACTGCTGCT Chr8:26059236..26059255 63.26 55
upstream ENSMUSE00000718304 Chr8:26059192..26059273 ATGCTGCACACACTGCTGCT Chr8:26059236..26059255 63.26 55
upstream ENSMUSE00000335046 Chr8:26048577..26048641 No primer for this exon
upstream ENSMUSE00000393886 Chr8:26034537..26034598 CACTGTGCACCTCCAGAAAA Chr8:26034538..26034557 59.87 50
upstream ENSMUSE00000408003 Chr8:26032732..26032807 No primer for this exon
upstream ENSMUSE00000353634 Chr8:26031760..26031836 CAAAGGCTACCCGAACTCAG Chr8:26031791..26031810 59.87 55

*** Putative Vector Insertion (Chr 8: 26031759 - 26032808) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000382993 Chr8:26030382..26030553 GCAGAGGCTCAATTCCGTAG Chr8:26030485..26030504 59.98 55
downstream ENSMUSE00000255155 Chr8:26026760..26026828 CGCTTTGTCCACCACAATACT Chr8:26026741..26026761 60.04 47.62
downstream ENSMUSE00000255149 Chr8:26024791..26024862 ATTGCTGTCAGAGCCCAAGT Chr8:26024814..26024833 59.87 50
downstream ENSMUSE00000255142 Chr8:26024431..26024597 CTCCAATGAGGACAACACGA Chr8:26024531..26024550 59.68 50
downstream ENSMUSE00000255132 Chr8:26013275..26013356 CTTCCCAGGATACGTTGCTC Chr8:26013289..26013308 59.69 55
downstream ENSMUSE00000255126 Chr8:26011914..26012050 GATGGATTCCGAGCAGTAGC Chr8:26011915..26011934 59.8 55
downstream ENSMUSE00000255116 Chr8:26008926..26009103 TGCTTCAGAGCCACAGTCAC Chr8:26008904..26008923 60.19 55
downstream ENSMUSE00000255108 Chr8:25996693..25996797 CAGCATGTTGATGAGGGAGA Chr8:25996684..25996703 59.79 50
downstream ENSMUSE00000340723 Chr8:25994910..25995108 TAGATGCTTTTCCGGCCTAC Chr8:25995045..25995064 59.32 50
downstream ENSMUSE00000386790 Chr8:25989193..25989301 ATAGCAGGCAAATGGAGCAT Chr8:25989251..25989270 59.7 45
downstream ENSMUSE00000343444 Chr8:25983044..25983233 TTGTAAGGCATTCGGGTAGG Chr8:25983158..25983177 59.95 50
downstream ENSMUSE00000383140 Chr8:25981158..25981241 TTCTACGCACACGCCATTTA Chr8:25981193..25981212 60.27 45
downstream ENSMUSE00000347940 Chr8:25974819..25974924 CAGTTGGGAGGTGAGTAGCC Chr8:25974847..25974866 59.72 60
downstream ENSMUSE00000387505 Chr8:25973861..25974017 GAGGAACACTGAAGCGAGGA Chr8:25973904..25973923 60.53 55
downstream ENSMUSE00000486334 Chr8:25962712..25962750 No primer for this exon
downstream ENSMUSE00000684800 Chr8:25962649..25962750 No primer for this exon
downstream ENSMUSE00000684799 Chr8:25962089..25962097 No primer for this exon
downstream ENSMUSE00000684804 Chr8:25948255..25948293 No primer for this exon
downstream ENSMUSE00000712522 Chr8:25948255..25948293 No primer for this exon
downstream ENSMUSE00000684803 Chr8:25946618..25946791 AGCTGTGACCAAGGGAAGAA Chr8:25946625..25946644 59.84 50
downstream ENSMUSE00000719508 Chr8:25946618..25946791 AGCTGTGACCAAGGGAAGAA Chr8:25946625..25946644 59.84 50
downstream ENSMUSE00000712485 Chr8:25946616..25946791 AGCTGTGACCAAGGGAAGAA Chr8:25946625..25946644 59.84 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGATCTTAATCGCCTTGCAG Chr8:26032743..26032764 60.72 47.62 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGATCTCGTGACTGGGAAAA Chr8:26032743..26032763 60.05 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000037437