Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI32413
Trapped Gene
Slc29a2 (ENSMUSG00000024891)
Vector Insertion
Chr 19: 5024593 - 5026027
Public Clones IST11884B6 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 8% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000253630 (Chr19:5024511..5024592 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGTTGGGATCAGCTTCTTCA Chr19:5024520..5024539 60.19 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000253630 (Chr19:5024511..5024592 +)
Downstram Exon
ENSMUSE00000253612 (Chr19:5026028..5026188 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGTTGGGATCAGCTTCTTCA Chr19:5024520..5024539 60.19 50 AAGGAGTTGAGGAGCGTGAA Chr19:5026179..5026198 59.99 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000145893 Chr19:5024071..5024277 AACGTGGTCCGCAGTGTAAC Chr19:5024086..5024105 61.02 55
upstream ENSMUSE00000253630 Chr19:5024511..5024592 GGTTGGGATCAGCTTCTTCA Chr19:5024520..5024539 60.19 50

*** Putative Vector Insertion (Chr 19: 5024593 - 5026027) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000253612 Chr19:5026028..5026188 AAGGAGTTGAGGAGCGTGAA Chr19:5026179..5026198 59.99 50
downstream ENSMUSE00000145878 Chr19:5026358..5026497 GAGCAGTAGTATGGCCAGCA Chr19:5026412..5026431 59.05 55
downstream ENSMUSE00000145904 Chr19:5027025..5027159 GGCCAAGGACATGAGCATAG Chr19:5027161..5027180 60.62 55
downstream ENSMUSE00000145875 Chr19:5027343..5027440 TACGATGGAGAGGAGGATGC Chr19:5027419..5027438 60.18 55
downstream ENSMUSE00000253525 Chr19:5027680..5027764 GAGACAGCTTCTCGGTCAGG Chr19:5027719..5027738 60.13 60
downstream ENSMUSE00000145876 Chr19:5028841..5028971 ATGGGAACCCCATTCTTCTC Chr19:5028865..5028884 60.13 50
downstream ENSMUSE00000145896 Chr19:5029212..5029320 AAAGACCGACAGGGTGACTG Chr19:5029271..5029290 60.15 55
downstream ENSMUSE00000145901 Chr19:5029409..5029494 TCCATGACGTTGAAGAGCAG Chr19:5029460..5029479 59.98 50
downstream ENSMUSE00000145898 Chr19:5030288..5030490 GAGCAGCATGAAGGTGATGA Chr19:5030440..5030459 59.95 50
downstream ENSMUSE00000344437 Chr19:5031002..5031963 CTTCCCCTCAGTTTCCATGA Chr19:5031583..5031602 60.04 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTTCTTCATTACCGCCATCC Chr19:5024572..5024592 59.53 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AAACCAACATCCCTCGTGAC Chr19:5024630..5024650 59.83 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024891