Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI32613
Trapped Gene
Rfx4 (ENSMUSG00000020037)
Vector Insertion
Chr 10: 84326133 - 84331096
Public Clones IST10512G8 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 52% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000641334 (Chr10:84325988..84326132 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000641334 (Chr10:84325988..84326132 +)
Downstram Exon
ENSMUSE00000641333 (Chr10:84331097..84331191 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000437360 Chr10:84218793..84219258 No primer for this exon
upstream ENSMUSE00000437352 Chr10:84242760..84242846 No primer for this exon
upstream ENSMUSE00000437349 Chr10:84261273..84261333 No primer for this exon
upstream ENSMUSE00000437343 Chr10:84277381..84277504 No primer for this exon
upstream ENSMUSE00000437341 Chr10:84298038..84298099 No primer for this exon
upstream ENSMUSE00000252574 Chr10:84300930..84301024 No primer for this exon
upstream ENSMUSE00000641342 Chr10:84302762..84302975 No primer for this exon
upstream ENSMUSE00000641339 Chr10:84303584..84303661 No primer for this exon
upstream ENSMUSE00000641338 Chr10:84306946..84307109 No primer for this exon
upstream ENSMUSE00000641337 Chr10:84321242..84321342 No primer for this exon
upstream ENSMUSE00000641336 Chr10:84323243..84323301 No primer for this exon
upstream ENSMUSE00000641334 Chr10:84325988..84326132 No primer for this exon

*** Putative Vector Insertion (Chr 10: 84326133 - 84331096) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000641333 Chr10:84331097..84331191 No primer for this exon
downstream ENSMUSE00000641332 Chr10:84331590..84331707 No primer for this exon
downstream ENSMUSE00000365299 Chr10:84334864..84335249 No primer for this exon
downstream ENSMUSE00000641331 Chr10:84342915..84343034 No primer for this exon
downstream ENSMUSE00000641330 Chr10:84343558..84343719 No primer for this exon
downstream ENSMUSE00000641328 Chr10:84356945..84357107 No primer for this exon
downstream ENSMUSE00000641327 Chr10:84358702..84358840 No primer for this exon
downstream ENSMUSE00000608931 Chr10:84367700..84369281 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTGAATTTGCCCAAAAGAC Chr10:84329163..84329183 59.69 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCTGAATTTGCCCAAAAGAC Chr10:84329163..84329183 59.69 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020037