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About
UniTrap UNI32805
Trapped Gene
Myo1f (
ENSMUSG00000024300
)
Vector Insertion
Chr 17: 33719382 - 33720629
Public Clones
IST14978A5 (tigm)
IST13670G2 (tigm)
IST13749A8 (tigm)
IST14978A5 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 33% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000395069 (Chr17:33719185..33719381 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
ACCAGCCGTAAGATGGACAG
Chr17:33719244..33719263
60.13
55
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000395069 (Chr17:33719185..33719381 +)
Downstram Exon
ENSMUSE00000140670 (Chr17:33720630..33720710 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
ACCAGCCGTAAGATGGACAG
Chr17:33719244..33719263
60.13
55
ACGCCGATGCTGTATTCTTC
Chr17:33720682..33720701
60.24
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000699210
Chr17:33692664..33692801
GAGGAACTAGGGCCAAGAGC
Chr17:33692682..33692701
60.35
60
upstream
ENSMUSE00000331991
Chr17:33712736..33712873
ACTGGCAGAGTCACAACGTG
Chr17:33712755..33712774
59.94
55
upstream
ENSMUSE00000140680
Chr17:33713379..33713468
CCTTCAAGCAGATGCCCTAC
Chr17:33713413..33713432
59.84
55
upstream
ENSMUSE00000546776
Chr17:33713558..33713652
CTCACCGACAACATGTACCG
Chr17:33713591..33713610
60.03
55
upstream
ENSMUSE00000400959
Chr17:33715038..33715125
CATCATGGGCTACATCTCCA
Chr17:33715077..33715096
59.48
50
upstream
ENSMUSE00000546771
Chr17:33715205..33715294
CAGTCAAACCCACTGCTTGA
Chr17:33715226..33715245
59.87
50
upstream
ENSMUSE00000546769
Chr17:33716812..33716943
GTTCAGTCGAGGTGGAGAGC
Chr17:33716832..33716851
59.99
60
upstream
ENSMUSE00000430762
Chr17:33717104..33717238
AAGGCACTGATGACCGAAGT
Chr17:33717201..33717220
59.73
50
upstream
ENSMUSE00000430749
Chr17:33718903..33719035
No primer for this exon
upstream
ENSMUSE00000395069
Chr17:33719185..33719381
ACCAGCCGTAAGATGGACAG
Chr17:33719244..33719263
60.13
55
*** Putative Vector Insertion (Chr 17: 33719382 - 33720629) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000140670
Chr17:33720630..33720710
ACGCCGATGCTGTATTCTTC
Chr17:33720682..33720701
60.24
50
downstream
ENSMUSE00000546764
Chr17:33720835..33720921
CCTTCAGCGTGAGCTCAATA
Chr17:33720916..33720935
59.17
50
downstream
ENSMUSE00000305523
Chr17:33722538..33722624
GCCCTCTTGCACATACTCCT
Chr17:33722561..33722580
59.31
55
downstream
ENSMUSE00000546729
Chr17:33723061..33723228
GCCTGCATAGTGATGGATGA
Chr17:33723228..33723247
59.63
50
downstream
ENSMUSE00000657679
Chr17:33725048..33725133
AGCACATCTCGATTCCTCTCA
Chr17:33725096..33725116
59.97
47.62
downstream
ENSMUSE00000657678
Chr17:33727773..33727854
CAGGAAAGAGCATCCGTAGG
Chr17:33727801..33727820
59.83
55
downstream
ENSMUSE00000546754
Chr17:33729866..33729972
AGTGGGGTGTGCACTTCTTC
Chr17:33729917..33729936
60.16
55
downstream
ENSMUSE00000372513
Chr17:33730966..33731064
CAGGTACTCCACCTGGTGCT
Chr17:33730993..33731012
60.17
60
downstream
ENSMUSE00000402427
Chr17:33731168..33731312
ATGTTGACAGCACGAAGCAG
Chr17:33731257..33731276
60.06
50
downstream
ENSMUSE00000140678
Chr17:33734886..33735000
CCAGGCTTTCTGGATAGTGC
Chr17:33734957..33734976
59.84
55
downstream
ENSMUSE00000546748
Chr17:33735088..33735257
ACTGAGTCGGCAAAGTCCAC
Chr17:33735235..33735254
60.31
55
downstream
ENSMUSE00000140687
Chr17:33737996..33738141
CGCACTAGACCCTTCTCTGG
Chr17:33738090..33738109
60.01
60
downstream
ENSMUSE00000140672
Chr17:33738604..33738750
CTCTTCAAAACGCTTGCACA
Chr17:33738715..33738734
60.17
45
downstream
ENSMUSE00000140684
Chr17:33738887..33739035
CTTTCTTCACCCGGAACTGT
Chr17:33738912..33738931
59.18
50
downstream
ENSMUSE00000140681
Chr17:33739471..33739554
No primer for this exon
downstream
ENSMUSE00000699190
Chr17:33739471..33739535
No primer for this exon
downstream
ENSMUSE00000699188
Chr17:33739538..33739554
No primer for this exon
downstream
ENSMUSE00000699187
Chr17:33739673..33739695
No primer for this exon
downstream
ENSMUSE00000140662
Chr17:33741202..33741397
GGTACCCCATTTCGATTCAA
Chr17:33741226..33741245
59.62
45
downstream
ENSMUSE00000699185
Chr17:33741202..33741254
GGTGGTACCCCATTTCGATT
Chr17:33741229..33741248
60.81
50
downstream
ENSMUSE00000699181
Chr17:33741272..33741316
No primer for this exon
downstream
ENSMUSE00000699180
Chr17:33741318..33741397
CCCTGGTCAGGCACATTAAG
Chr17:33741391..33741410
60.51
55
downstream
ENSMUSE00000140665
Chr17:33741495..33741664
No primer for this exon
downstream
ENSMUSE00000546724
Chr17:33744244..33744703
CTGGGAGGTAGGTTTTGCAG
Chr17:33744572..33744591
59.73
55
downstream
ENSMUSE00000707337
Chr17:33744244..33744320
CTTCCACCAGCCAGAAGAGT
Chr17:33744266..33744285
59.45
55
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GGTGGAGGTGAGTAGGACCA
Chr17:33719376..33719396
59.96
60
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GGTGGAGGTGAGTAGGACCA
Chr17:33719376..33719396
59.96
60
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000024300