Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI32878
Trapped Gene
Smyd4 (
ENSMUSG00000018809
)
Vector Insertion
Chr 11: 75195897 - 75200912
Public Clones
(cmhd)
IST14987G3 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 12% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000387423 (Chr11:75195752..75195896 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000387423 (Chr11:75195752..75195896 +)
Downstram Exon
ENSMUSE00000347031 (Chr11:75200913..75201002 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000443502
Chr11:75162000..75162253
No primer for this exon
upstream
ENSMUSE00000333804
Chr11:75163171..75163316
No primer for this exon
upstream
ENSMUSE00000387423
Chr11:75195752..75195896
No primer for this exon
*** Putative Vector Insertion (Chr 11: 75195897 - 75200912) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000347031
Chr11:75200913..75201002
No primer for this exon
downstream
ENSMUSE00000380554
Chr11:75203574..75204729
No primer for this exon
downstream
ENSMUSE00000110532
Chr11:75213103..75213285
No primer for this exon
downstream
ENSMUSE00000110531
Chr11:75213784..75213947
No primer for this exon
downstream
ENSMUSE00000110533
Chr11:75215620..75215752
No primer for this exon
downstream
ENSMUSE00000110530
Chr11:75216611..75216727
No primer for this exon
downstream
ENSMUSE00000299789
Chr11:75216919..75217042
No primer for this exon
downstream
ENSMUSE00000338606
Chr11:75218268..75219207
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TCACTTCCAGCCCCATAATC
Chr11:75198933..75198953
59.89
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GTCCAACCAGCAGTTGAACA
Chr11:75198907..75198927
59.73
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000018809