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About
UniTrap UNI32901
Trapped Gene
Pkd1l1 (
ENSMUSG00000046634
)
Vector Insertion
Chr 11: 8816249 - 8829479
Public Clones
(sanger)
(sanger)
IST14740H7 (tigm)
IST10885F2 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 38% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000581114 (Chr11:8829358..8829478 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CCTGTACCGTGGTCAAGACA
Chr11:8829409..8829428
59.59
55
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000581114 (Chr11:8829358..8829478 -)
Downstram Exon
ENSMUSE00000581113 (Chr11:8816250..8816361 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CCTGTACCGTGGTCAAGACA
Chr11:8829409..8829428
59.59
55
ATGGTAACGGGGTAGCACAG
Chr11:8816254..8816273
59.88
55
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000581129
Chr11:8872947..8873268
GGCAGGTGCAACTACTGTGA
Chr11:8872971..8872990
59.9
55
upstream
ENSMUSE00000581128
Chr11:8865052..8865219
CTCAAGGCTGTTGTCCATGA
Chr11:8865186..8865205
59.83
50
upstream
ENSMUSE00000581127
Chr11:8862597..8862716
CAGTCGTGGTGCATTGTTTT
Chr11:8862694..8862713
59.62
45
upstream
ENSMUSE00000581126
Chr11:8861929..8862097
GGAGTTCGCTTGGTATTTCG
Chr11:8861993..8862012
59.71
50
upstream
ENSMUSE00000581125
Chr11:8861260..8861499
TCTCTGAACCCCACCTCATC
Chr11:8861436..8861455
60.05
55
upstream
ENSMUSE00000581124
Chr11:8858854..8858986
AACCACCCCCAGTGAAGAAC
Chr11:8858875..8858894
61.18
55
upstream
ENSMUSE00000581123
Chr11:8853865..8854071
CATCATGCTCCCAAGCTACA
Chr11:8853900..8853919
59.82
50
upstream
ENSMUSE00000581122
Chr11:8850352..8850533
CGTCATCTCTGAAGGCACAC
Chr11:8850440..8850459
59.42
55
upstream
ENSMUSE00000581121
Chr11:8847148..8847369
TGGCCCTGCTTTGATAACTC
Chr11:8847313..8847332
60.21
50
upstream
ENSMUSE00000581120
Chr11:8845188..8845348
TCCCAGACCTCACGTATTCC
Chr11:8845234..8845253
59.93
55
upstream
ENSMUSE00000581119
Chr11:8841905..8842246
TTGGGACCAGTCTGAAGTCC
Chr11:8842185..8842204
60.09
55
upstream
ENSMUSE00000581118
Chr11:8841044..8841103
TAGCGACATCCAAGAAGCAG
Chr11:8841068..8841087
59.17
50
upstream
ENSMUSE00000581117
Chr11:8836848..8837024
CCAGTTATGGGGACAACCTG
Chr11:8836951..8836970
60.22
55
upstream
ENSMUSE00000581116
Chr11:8833643..8833720
TCACTGAGCAGTGGCAAGAC
Chr11:8833666..8833685
60.19
55
upstream
ENSMUSE00000581115
Chr11:8831552..8831694
GCATGTCTTACTGGGCAAGG
Chr11:8831668..8831687
60.66
55
upstream
ENSMUSE00000581114
Chr11:8829358..8829478
CCTGTACCGTGGTCAAGACA
Chr11:8829409..8829428
59.59
55
upstream
ENSMUSE00000581113
Chr11:8816250..8816361
CTGTGCTACCCCGTTACCAT
Chr11:8816276..8816295
59.88
55
*** Putative Vector Insertion (Chr 11: 8816249 - 8829479) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000581112
Chr11:8809804..8810002
ACATACAAGCGGCTCAGGAT
Chr11:8809873..8809892
59.72
50
downstream
ENSMUSE00000581111
Chr11:8808672..8808728
No primer for this exon
downstream
ENSMUSE00000581110
Chr11:8802412..8802621
TCCTGCAGATAGTCCCCATC
Chr11:8802424..8802443
60.03
55
downstream
ENSMUSE00000581109
Chr11:8801145..8801360
CTCTGAGGGCTACGATGGAG
Chr11:8801280..8801299
59.97
60
downstream
ENSMUSE00000581108
Chr11:8790660..8790770
GTCTTCTTCCCCAAACTCCA
Chr11:8790644..8790663
59.11
50
downstream
ENSMUSE00000581107
Chr11:8790391..8790545
ACATTCACCTCATCCCGAAG
Chr11:8790474..8790493
59.93
50
downstream
ENSMUSE00000581106
Chr11:8789030..8789130
GACCCGCTTCACAAGAAAAT
Chr11:8789063..8789082
59.17
45
downstream
ENSMUSE00000581105
Chr11:8786576..8786768
ACGATCCTGGCTGTGGATAG
Chr11:8786576..8786595
60.1
55
downstream
ENSMUSE00000371892
Chr11:8781401..8781484
CAGAGACAGGTGCAAGGTGA
Chr11:8781437..8781456
60.02
55
downstream
ENSMUSE00000373525
Chr11:8779103..8779310
AAAATACCGGGGAGCAAGTT
Chr11:8779259..8779278
59.83
45
downstream
ENSMUSE00000337982
Chr11:8775655..8775761
GCCTGGAATTCCTTCCAAAT
Chr11:8775649..8775668
60.26
45
downstream
ENSMUSE00000373117
Chr11:8774102..8774318
AGAGGCGGATCTTCTGGAGT
Chr11:8774256..8774275
60.36
55
downstream
ENSMUSE00000354314
Chr11:8771707..8771880
TGTTGATGGTCTCGCACAGT
Chr11:8771686..8771705
60.32
50
downstream
ENSMUSE00000402878
Chr11:8770208..8770327
GACAGAAGCTGTGCAACTGG
Chr11:8770208..8770227
59.62
55
downstream
ENSMUSE00000362346
Chr11:8770052..8770121
CACTGTGTTGAAGCCTGCAT
Chr11:8770065..8770084
59.9
50
downstream
ENSMUSE00000399659
Chr11:8769461..8769504
GCTGCCTTGAATCTGGTCTC
Chr11:8769447..8769466
59.96
55
downstream
ENSMUSE00000336750
Chr11:8768460..8768616
ATTGGGAACGGTGAACAGAC
Chr11:8768506..8768525
59.83
50
downstream
ENSMUSE00000387886
Chr11:8767134..8767269
CTGGGACCCAAGTTCTTCAA
Chr11:8767213..8767232
60.08
50
downstream
ENSMUSE00000344862
Chr11:8766095..8766179
AGCCACCACATACACTGTGC
Chr11:8766128..8766147
59.63
55
downstream
ENSMUSE00000368126
Chr11:8765485..8765661
GAACACGGGTTCTGGAATGT
Chr11:8765513..8765532
59.83
50
downstream
ENSMUSE00000352032
Chr11:8763806..8763924
GAGCTTAGCCTTGGATGGTG
Chr11:8763837..8763856
59.84
55
downstream
ENSMUSE00000488867
Chr11:8761199..8761309
TTTCCTGATGGCATGATTGA
Chr11:8761188..8761207
60.01
40
downstream
ENSMUSE00000477426
Chr11:8757039..8757159
TCCAGTCCCACCAGTCTTCT
Chr11:8757082..8757101
59.68
55
downstream
ENSMUSE00000462946
Chr11:8756263..8756346
GCAGTGCCAGCAGAAATCTT
Chr11:8756248..8756267
60.55
50
downstream
ENSMUSE00000465514
Chr11:8755566..8755714
CAGGGGACACATTTTGGTTC
Chr11:8755605..8755624
60.21
50
downstream
ENSMUSE00000466504
Chr11:8754346..8754580
CAGGATCACGCTTGTGAAGA
Chr11:8754432..8754451
59.98
50
downstream
ENSMUSE00000483403
Chr11:8749658..8749762
GGAAACAGAGGTGGATCACG
Chr11:8749701..8749720
60.51
55
downstream
ENSMUSE00000581103
Chr11:8748921..8749061
GAACTGGTCGGTGATGTTCA
Chr11:8748956..8748975
59.53
50
downstream
ENSMUSE00000581102
Chr11:8744544..8744669
CACTGCTGAGAAGGGTGTCA
Chr11:8744549..8744568
60.02
55
downstream
ENSMUSE00000581101
Chr11:8736206..8736436
CCGTGGTCAGACTTCAAACA
Chr11:8736245..8736264
59.72
50
downstream
ENSMUSE00000581100
Chr11:8734777..8734935
CCCAGAAGGGTGAGGACTTT
Chr11:8734798..8734817
60.48
55
downstream
ENSMUSE00000581099
Chr11:8733777..8733947
AACTGCCAACTGGAGGACTG
Chr11:8733775..8733794
60.3
55
downstream
ENSMUSE00000581098
Chr11:8726728..8726810
CAGCAGCTAAGGGACCAGAT
Chr11:8726767..8726786
59.45
55
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TATAATCGCCTTGCAGCACAT
Chr11:8823409..8823430
60.62
42.86
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TCACATACGTGACTGGGAAAA
Chr11:8823414..8823435
59.04
42.86
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000046634