Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI33157
Trapped Gene
AC158617.9 (ENSMUSG00000019767)
Vector Insertion
Chr 10: 5812264 - 5825787
Public Clones IST12093G2 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 36% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000318476 (Chr10:5825601..5825786 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000318476 (Chr10:5825601..5825786 -)
Downstram Exon
ENSMUSE00000318468 (Chr10:5812265..5812582 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000318508 Chr10:5833661..5833846 No primer for this exon
upstream ENSMUSE00000318492 Chr10:5832229..5832485 No primer for this exon
upstream ENSMUSE00000318484 Chr10:5827491..5827635 No primer for this exon
upstream ENSMUSE00000318476 Chr10:5825601..5825786 No primer for this exon
upstream ENSMUSE00000318468 Chr10:5812265..5812582 No primer for this exon

*** Putative Vector Insertion (Chr 10: 5812264 - 5825787) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000318459 Chr10:5804659..5804859 No primer for this exon
downstream ENSMUSE00000318445 Chr10:5799602..5799775 No primer for this exon
downstream ENSMUSE00000306663 Chr10:5796742..5796984 No primer for this exon
downstream ENSMUSE00000306622 Chr10:5787808..5788044 No primer for this exon
downstream ENSMUSE00000341294 Chr10:5785538..5785642 No primer for this exon
downstream ENSMUSE00000577430 Chr10:5785430..5785510 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGGGAATTGCAGGGTTTAT Chr10:5819760..5819780 58.9 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAGTAGGTCGTGACTGGGAAA Chr10:5825723..5825744 60.16 52.38 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000019767