Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI33241
Trapped Gene
A430107P09Rik (ENSMUSG00000076928)
Vector Insertion
Chr 14: 54840457 - 54841780
Public Clones IST13942G3 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 63% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000663890 (Chr14:54840196..54840456 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AAATCAATGTGCCGAAAACC Chr14:54840286..54840305 59.81 40 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000663890 (Chr14:54840196..54840456 +)
Downstram Exon
ENSMUSE00000663888 (Chr14:54841781..54841825 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AAATCAATGTGCCGAAAACC Chr14:54840286..54840305 59.81 40 TTCTCAGTCAACGTGGCATC Chr14:54841814..54841833 59.84 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000663890 Chr14:54840196..54840456 AAATCAATGTGCCGAAAACC Chr14:54840286..54840305 59.81 40

*** Putative Vector Insertion (Chr 14: 54840457 - 54841780) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000663888 Chr14:54841781..54841825 TTCTCAGTCAACGTGGCATC Chr14:54841814..54841833 59.84 50
downstream ENSMUSE00000663889 Chr14:54842666..54842772 AAATCCGGCTACTTTCAGCA Chr14:54842739..54842758 59.85 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATGGTTTCCAACGTGACTGG Chr14:54840497..54840517 60.81 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000076928