Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI33285
Trapped Gene
Pvrl2 (ENSMUSG00000062300)
Vector Insertion
Chr 7: 20302014 - 20304632
Public Clones PST22881-NR (escells) IST12320G4 (tigm) IST12823F5 (tigm) IST14170E2 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000198357 (Chr7:20304545..20304631 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GACGCCATACTTTGATGCTG Chr7:20304565..20304584 59.3 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000198357 (Chr7:20304545..20304631 -)
Downstram Exon
ENSMUSE00000485480 (Chr7:20302015..20303136 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GACGCCATACTTTGATGCTG Chr7:20304565..20304584 59.3 50 CAGATGCCCAGATTCTCCAT Chr7:20302047..20302066 60.03 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000536242 Chr7:20334564..20334818 CCGATCGGTACACGAAGAGT Chr7:20334700..20334719 60.13 55
upstream ENSMUSE00000357931 Chr7:20323361..20323723 GGCAATTACACCTGCGAGTT Chr7:20323414..20323433 60.14 50
upstream ENSMUSE00000198358 Chr7:20318264..20318560 CCGAATCACCTGGATCTCAT Chr7:20318444..20318463 59.89 50
upstream ENSMUSE00000198353 Chr7:20315961..20316078 ATCCATCTCCGGCTATGATG Chr7:20316046..20316065 59.88 50
upstream ENSMUSE00000198361 Chr7:20315387..20315535 TCTGTGGATCGAATGGTCAA Chr7:20315458..20315477 60.05 45
upstream ENSMUSE00000676814 Chr7:20309830..20310218 TCATGCTACCTCCACACGAG Chr7:20309899..20309918 59.85 55
upstream ENSMUSE00000198359 Chr7:20306702..20306855 TATTATCGCTGCCATCATCG Chr7:20306787..20306806 59.64 45
upstream ENSMUSE00000198354 Chr7:20304782..20304845 TGGAAGGACCTCCCTCCTAT Chr7:20304824..20304843 59.89 55
upstream ENSMUSE00000198357 Chr7:20304545..20304631 GACGCCATACTTTGATGCTG Chr7:20304565..20304584 59.3 50
upstream ENSMUSE00000485480 Chr7:20302015..20303136 CATTGCAATCCACCTGTGAC Chr7:20302736..20302755 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000062300