Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI33388
Trapped Gene
Aes (ENSMUSG00000054452)
Vector Insertion
Chr 10: 81022414 - 81023922
Public Clones IST12122D7 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000641506 (Chr10:81022348..81022413 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CGCGACTGACATGATGTTTC Chr10:81022377..81022396 60.27 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000641506 (Chr10:81022348..81022413 +)
Downstram Exon
ENSMUSE00000665809 (Chr10:81023923..81023964 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CGCGACTGACATGATGTTTC Chr10:81022377..81022396 60.27 50 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000641506 Chr10:81022348..81022413 CGCGACTGACATGATGTTTC Chr10:81022377..81022396 60.27 50

*** Putative Vector Insertion (Chr 10: 81022414 - 81023922) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000665809 Chr10:81023923..81023964 No primer for this exon
downstream ENSMUSE00000298746 Chr10:81023964..81024061 ACTCATCTTTGATGCGGTCA Chr10:81024036..81024055 59.24 45
downstream ENSMUSE00000665808 Chr10:81023967..81024061 AGTCGGAGGTGGTGAACTTG Chr10:81024012..81024031 60.15 55
downstream ENSMUSE00000574607 Chr10:81026840..81026903 TGACGTAATGCCTCTGCATC Chr10:81026904..81026923 59.83 50
downstream ENSMUSE00000574606 Chr10:81027418..81027462 TGCATCTCGATGTTCAATCC Chr10:81027458..81027477 59.61 45
downstream ENSMUSE00000298726 Chr10:81027593..81027655 ATCCCATTCAGCCTCTTCAC Chr10:81027624..81027643 59.09 50
downstream ENSMUSE00000102269 Chr10:81028037..81028111 GTGACCTGCTTGGCTCTCTC Chr10:81028086..81028105 60.14 60
downstream ENSMUSE00000368559 Chr10:81028217..81028909 CCTTCCCCAAGAGCTAATCC Chr10:81028454..81028473 60.03 55
downstream ENSMUSE00000705336 Chr10:81028217..81028438 AATCCGACTTCTCTCCGTCA Chr10:81028439..81028458 59.8 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC Chr10:81022465..81022485 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000054452