Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI33433
Trapped Gene
E2f4 (ENSMUSG00000014859)
Vector Insertion
Chr 8: 107821776 - 107822180
Public Clones IST14976H11 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 11% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000336331 (Chr8:107821617..107821775 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000336331 (Chr8:107821617..107821775 +)
Downstram Exon
ENSMUSE00000214314 (Chr8:107822181..107822290 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000336331 Chr8:107821617..107821775 No primer for this exon

*** Putative Vector Insertion (Chr 8: 107821776 - 107822180) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000214314 Chr8:107822181..107822290 No primer for this exon
downstream ENSMUSE00000214319 Chr8:107822435..107822596 No primer for this exon
downstream ENSMUSE00000214320 Chr8:107822896..107822939 No primer for this exon
downstream ENSMUSE00000214318 Chr8:107823958..107824019 No primer for this exon
downstream ENSMUSE00000214316 Chr8:107824219..107824516 No primer for this exon
downstream ENSMUSE00000361381 Chr8:107825153..107825365 No primer for this exon
downstream ENSMUSE00000235286 Chr8:107828085..107828132 No primer for this exon
downstream ENSMUSE00000235261 Chr8:107828333..107828377 No primer for this exon
downstream ENSMUSE00000459478 Chr8:107828473..107829267 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTAATCGCCTTGCAGCACAT Chr8:107821826..107821846 61.2 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTCTTGCAGGAAGCCAAGGAC Chr8:107821735..107821756 63.58 57.14 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000014859