Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI33603
Trapped Gene
Angptl4 (ENSMUSG00000002289)
Vector Insertion
Chr 17: 33917727 - 33918489
Public Clones IST13740G7 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000303846 (Chr17:33918008..33918488 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000303846 (Chr17:33918008..33918488 -)
Downstram Exon
ENSMUSE00000140709 (Chr17:33917728..33917838 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000303846 Chr17:33918008..33918488 No primer for this exon
upstream ENSMUSE00000140709 Chr17:33917728..33917838 No primer for this exon

*** Putative Vector Insertion (Chr 17: 33917727 - 33918489) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000140707 Chr17:33917475..33917592 No primer for this exon
downstream ENSMUSE00000140710 Chr17:33915317..33915430 No primer for this exon
downstream ENSMUSE00000140705 Chr17:33914265..33914360 No primer for this exon
downstream ENSMUSE00000140706 Chr17:33913895..33914176 No primer for this exon
downstream ENSMUSE00000303676 Chr17:33911849..33912513 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTATGAGCTACGGGCTCCAG Chr17:33918460..33918480 60.37 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACGGGCTCCAGATCTTCTTC Chr17:33918451..33918471 60.74 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000002289