Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI3376
Trapped Gene
Etv5 (ENSMUSG00000013089)
Vector Insertion
Chr 16: 22386932 - 22392727
Public Clones AW0415 (sanger) XR0283 (sanger) XR0275 (sanger)
Private Clones OST398358 (lexicon) OST339457 (lexicon) OST211859 (lexicon)
Severity of mutation (?) Insertion after 79% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000131370 (Chr16:22392728..22392897 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000131370 (Chr16:22392728..22392897 -)
Downstram Exon
ENSMUSE00000560895 (Chr16:22386830..22386931 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000560923 Chr16:22439590..22439691 No primer for this exon
upstream ENSMUSE00000407999 Chr16:22436511..22436633 No primer for this exon
upstream ENSMUSE00000378517 Chr16:22436274..22436361 No primer for this exon
upstream ENSMUSE00000310554 Chr16:22436093..22436140 No primer for this exon
upstream ENSMUSE00000478899 Chr16:22435950..22436000 No primer for this exon
upstream ENSMUSE00000644755 Chr16:22421892..22421945 No primer for this exon
upstream ENSMUSE00000414095 Chr16:22412918..22413047 No primer for this exon
upstream ENSMUSE00000497015 Chr16:22411706..22411993 No primer for this exon
upstream ENSMUSE00000362934 Chr16:22401745..22402004 No primer for this exon
upstream ENSMUSE00000310460 Chr16:22399393..22399452 No primer for this exon
upstream ENSMUSE00000310522 Chr16:22393060..22393128 No primer for this exon
upstream ENSMUSE00000131370 Chr16:22392728..22392897 No primer for this exon

*** Putative Vector Insertion (Chr 16: 22386932 - 22392727) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000560895 Chr16:22386830..22386931 No primer for this exon
downstream ENSMUSE00000702629 Chr16:22381385..22383764 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAACCGGAAGAGGTGAGTCC Chr16:22389718..22389738 61.03 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGGGGACAGGGTTGTATGT Chr16:22392697..22392717 60.49 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000013089