Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI34088
Trapped Gene
Tmem134 (ENSMUSG00000024845)
Vector Insertion
Chr 19: 4126201 - 4127056
Public Clones IST13219C10 (tigm) IST14205H4 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 31% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000554572 (Chr19:4125987..4126200 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GTTCGAGGTGGCTGATGAAG Chr19:4126155..4126174 60.8 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000554572 (Chr19:4125987..4126200 +)
Downstram Exon
ENSMUSE00000380485 (Chr19:4127057..4127121 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GTTCGAGGTGGCTGATGAAG Chr19:4126155..4126174 60.8 55 GCTCCGTCCTCATCATTTTC Chr19:4127085..4127104 59.63 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000554572 Chr19:4125987..4126200 GTTCGAGGTGGCTGATGAAG Chr19:4126155..4126174 60.8 55

*** Putative Vector Insertion (Chr 19: 4126201 - 4127056) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000380485 Chr19:4127057..4127121 GCTCCGTCCTCATCATTTTC Chr19:4127085..4127104 59.63 50
downstream ENSMUSE00000485451 Chr19:4127550..4127639 No primer for this exon
downstream ENSMUSE00000145490 Chr19:4127760..4127836 GCGGTTTTTCTGGATCAAAG Chr19:4127796..4127815 59.69 45
downstream ENSMUSE00000434717 Chr19:4131031..4131075 No primer for this exon
downstream ENSMUSE00000644532 Chr19:4131229..4131282 GGCACAAAGAAGATGGCACT Chr19:4131259..4131278 60.26 50
downstream ENSMUSE00000472768 Chr19:4131362..4131727 CCAGGTGTAGGTTGGAGGAA Chr19:4131534..4131553 59.96 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGGCTGATGAAGACAAGCAG Chr19:4126164..4126184 60.14 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGGCTGATGAAGACAAGCAG Chr19:4126164..4126184 60.14 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024845