Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI34123
Trapped Gene
Mospd3 (ENSMUSG00000037221)
Vector Insertion
Chr 5: 138041498 - 138042273
Public Clones IST10911H5 (tigm) IST10911H5 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000387892 (Chr5:138041970..138042272 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AAGAGCAGAGTCCGTCAGGA Chr5:138042083..138042102 60.13 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000387892 (Chr5:138041970..138042272 -)
Downstram Exon
ENSMUSE00000686289 (Chr5:138041499..138041936 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AAGAGCAGAGTCCGTCAGGA Chr5:138042083..138042102 60.13 55 CTGGTCCGCCCTGAATACTA Chr5:138041541..138041560 60.09 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000387892 Chr5:138041970..138042272 AAGAGCAGAGTCCGTCAGGA Chr5:138042083..138042102 60.13 55
upstream ENSMUSE00000343999 Chr5:138041499..138041771 TAGTATTCAGGGCGGACCAG Chr5:138041563..138041582 60.09 55
upstream ENSMUSE00000686289 Chr5:138041499..138041936 TAGTATTCAGGGCGGACCAG Chr5:138041563..138041582 60.09 55

*** Putative Vector Insertion (Chr 5: 138041498 - 138042273) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000306984 Chr5:138041205..138041280 TACCGTGTATTTGGCAGGTG Chr5:138041227..138041246 59.46 50
downstream ENSMUSE00000306979 Chr5:138040822..138041051 CTCAATGCGGAATCTGTCCT Chr5:138040966..138040985 60.22 50
downstream ENSMUSE00000306973 Chr5:138038379..138038543 GCAGTGGTAGGAGCAGGAAG Chr5:138038435..138038454 60.01 60
downstream ENSMUSE00000686293 Chr5:138037884..138038281 AGGAGCAAGGTGCAAACATC Chr5:138038143..138038162 60.26 50
downstream ENSMUSE00000379307 Chr5:138037873..138038281 AGGAGCAAGGTGCAAACATC Chr5:138038143..138038162 60.26 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTTTGACCTAATCGCCTTGC Chr5:138042210..138042230 59.85 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGACCGGAGGAAACTTTGAC Chr5:138042223..138042243 60.09 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000037221