Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI34193
Trapped Gene
Akap10 (ENSMUSG00000047804)
Vector Insertion
Chr 11: 61729585 - 61736332
Public Clones IST12378E8 (tigm) IST12002E11 (tigm) IST12393E2 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 8% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000431729 (Chr11:61736284..61736331 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000431729 (Chr11:61736284..61736331 -)
Downstram Exon
ENSMUSE00000431724 (Chr11:61729586..61729768 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon GGCTGTTTCTTAAGCGTTGC Chr11:61729599..61729618 60.03 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000662191 Chr11:61743491..61743728 AAGGGGCTGTTTTCGGACTA Chr11:61743623..61743642 60.98 50
upstream ENSMUSE00000717396 Chr11:61743491..61743728 AAGGGGCTGTTTTCGGACTA Chr11:61743623..61743642 60.98 50
upstream ENSMUSE00000431729 Chr11:61736284..61736331 No primer for this exon
upstream ENSMUSE00000431724 Chr11:61729586..61729768 ACCAAGTCATGTTGCGATCA Chr11:61729685..61729704 60.12 45
upstream ENSMUSE00000677738 Chr11:61729586..61729720 ACCAAGTCATGTTGCGATCA Chr11:61729685..61729704 60.12 45

*** Putative Vector Insertion (Chr 11: 61729585 - 61736332) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000345045 Chr11:61728526..61729083 CCAAAATTTCACCAGGTGCT Chr11:61728916..61728935 59.97 45
downstream ENSMUSE00000353594 Chr11:61723350..61723448 TTGGTATTGGCTTAGCAGCAT Chr11:61723358..61723378 59.76 42.86
downstream ENSMUSE00000399914 Chr11:61718277..61718361 GTGCCAGAACGAAACAGTTG Chr11:61718286..61718305 59.34 50
downstream ENSMUSE00000388091 Chr11:61717409..61717532 TGGTCAGCACTTCAATCTGG Chr11:61717449..61717468 59.83 50
downstream ENSMUSE00000413489 Chr11:61713812..61713948 GGAAATTATCCGCTGCTAACC Chr11:61713866..61713886 59.95 47.62
downstream ENSMUSE00000399981 Chr11:61710150..61710294 TTGTCCAGGCCTGACGTAAT Chr11:61710139..61710158 60.52 50
downstream ENSMUSE00000387852 Chr11:61706882..61707055 ATCGGAACCACCTGAGTGAG Chr11:61706875..61706894 60.11 55
downstream ENSMUSE00000677736 Chr11:61705279..61707055 ACTCCTCAGGAAGGCAGACA Chr11:61706895..61706914 59.99 55
downstream ENSMUSE00000349923 Chr11:61703740..61703849 TTCTGGGTCCAGACTTGCAG Chr11:61703744..61703763 61.39 55
downstream ENSMUSE00000677737 Chr11:61703292..61703849 GGCAGGGAATTCACTATGGA Chr11:61703510..61703529 59.89 50
downstream ENSMUSE00000359233 Chr11:61700202..61700284 AGGCTCAGACTCTCGGATGA Chr11:61700205..61700224 60.1 55
downstream ENSMUSE00000404737 Chr11:61691476..61691528 CCTTGCACCCACTTCTTCAT Chr11:61691467..61691486 60.11 50
downstream ENSMUSE00000662190 Chr11:61690809..61690904 AGTGGTTGATCATGGTGTGC Chr11:61690803..61690822 59.4 50
downstream ENSMUSE00000662189 Chr11:61684809..61686523 GATAGCGGAATGGGTCTTGA Chr11:61685844..61685863 60.04 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACTTAATCGCCTTGCAGCAC Chr11:61730264..61730284 60.42 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000047804