Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI3420
Trapped Gene
Tmem49 (ENSMUSG00000018171)
Vector Insertion
Chr 11: 86424925 - 86448676
Public Clones AB0036 (sanger) AB0125 (sanger) NAISTrap_18v3017 (NAISTrap) IST13227D8 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 59% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000105547 (Chr11:86448677..86448808 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000105547 (Chr11:86448677..86448808 -)
Downstram Exon
ENSMUSE00000105546 (Chr11:86424844..86424924 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000392078 Chr11:86497242..86497323 No primer for this exon
upstream ENSMUSE00000105552 Chr11:86479279..86479380 No primer for this exon
upstream ENSMUSE00000105551 Chr11:86478156..86478291 No primer for this exon
upstream ENSMUSE00000105557 Chr11:86476985..86477075 No primer for this exon
upstream ENSMUSE00000105555 Chr11:86474625..86474735 No primer for this exon
upstream ENSMUSE00000105549 Chr11:86457000..86457167 No primer for this exon
upstream ENSMUSE00000105547 Chr11:86448677..86448808 No primer for this exon

*** Putative Vector Insertion (Chr 11: 86424925 - 86448676) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000105546 Chr11:86424844..86424924 No primer for this exon
downstream ENSMUSE00000105556 Chr11:86420678..86420794 No primer for this exon
downstream ENSMUSE00000105554 Chr11:86415510..86415571 No primer for this exon
downstream ENSMUSE00000105558 Chr11:86399998..86400100 No primer for this exon
downstream ENSMUSE00000363556 Chr11:86398198..86398953 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TACCACCACCATGCAAGACT Chr11:86427700..86427720 59.01 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TACCACCACCATGCAAGACT Chr11:86427700..86427720 59.01 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 GCTTCCTATGGCCTTGAACA Chr11:86427832..86427852 60.21 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 GCTTCCTATGGCCTTGAACA Chr11:86427832..86427852 60.21 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018171