Tools for the biologist enabling optimized use of gene trap clones
Homepage
|
Blast Search
|
GO Search
|
Advanced Search
|
About
UniTrap UNI34236
Trapped Gene
Gabbr1 (
ENSMUSG00000024462
)
Vector Insertion
Chr 17: 37183722 - 37184398
Public Clones
IST11302A9 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000543755 (Chr17:37183640..37183721 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000543755 (Chr17:37183640..37183721 +)
Downstram Exon
ENSMUSE00000448950 (Chr17:37184399..37184602 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
TGTCCATATCCGTCCAGGAG
Chr17:37184590..37184609
60.88
55
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000696575
Chr17:37183016..37183234
AGCCTGGATTCCTGTGGAAG
Chr17:37183105..37183124
61.55
55
upstream
ENSMUSE00000543755
Chr17:37183640..37183721
No primer for this exon
*** Putative Vector Insertion (Chr 17: 37183722 - 37184398) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000448950
Chr17:37184399..37184602
TGTCCATATCCGTCCAGGAG
Chr17:37184590..37184609
60.88
55
downstream
ENSMUSE00000503591
Chr17:37185366..37185551
GGCCCTGACTACAGATGCTC
Chr17:37185524..37185543
59.83
60
downstream
ENSMUSE00000696573
Chr17:37186722..37186742
No primer for this exon
downstream
ENSMUSE00000696551
Chr17:37187750..37188104
TAGTCCGGCAGGATGTCTCT
Chr17:37188076..37188095
59.83
55
downstream
ENSMUSE00000510627
Chr17:37187944..37188104
TAGTCCGGCAGGATGTCTCT
Chr17:37188076..37188095
59.83
55
downstream
ENSMUSE00000142512
Chr17:37191076..37191210
GATCTTGATGGGGTCGTTGT
Chr17:37191141..37191160
59.79
50
downstream
ENSMUSE00000142518
Chr17:37191682..37191852
GTCCGAAAGAACGTTGGAAA
Chr17:37191749..37191768
60.09
45
downstream
ENSMUSE00000142524
Chr17:37192791..37192892
TGATCTCAATCCCAGCCTCT
Chr17:37192842..37192861
59.76
50
downstream
ENSMUSE00000142505
Chr17:37193241..37193306
AAGTCCCACGATGATTCGAG
Chr17:37193273..37193292
60.07
50
downstream
ENSMUSE00000142507
Chr17:37193733..37193924
CCCAAAGAGCCGTTCCTTAT
Chr17:37193759..37193778
60.44
50
downstream
ENSMUSE00000142508
Chr17:37199479..37199721
CTTTTCAGCCGCTTGGTTAG
Chr17:37199525..37199544
60.01
50
downstream
ENSMUSE00000142525
Chr17:37200275..37200338
GCTGCTCGATAAGCGTCCAT
Chr17:37200335..37200354
62.72
55
downstream
ENSMUSE00000142511
Chr17:37201605..37201682
ATCATCCTTGGTGCTGTCGT
Chr17:37201654..37201673
60.54
50
downstream
ENSMUSE00000142515
Chr17:37204159..37204309
GGCTGGAGAGAACTGAGACG
Chr17:37204254..37204273
60.13
60
downstream
ENSMUSE00000142510
Chr17:37204721..37204853
GACAGCAGCTAGTGCCAGTG
Chr17:37204796..37204815
59.8
60
downstream
ENSMUSE00000142522
Chr17:37206057..37206173
TAAAGCCTAAGCCCAAGAGC
Chr17:37206090..37206109
58.74
50
downstream
ENSMUSE00000142517
Chr17:37206258..37206365
GGCCTACAGTGGCGTACAGT
Chr17:37206297..37206316
60.2
60
downstream
ENSMUSE00000142521
Chr17:37206926..37207019
GGTTCCTCCTTGGCAAAAGT
Chr17:37206948..37206967
60.48
50
downstream
ENSMUSE00000696549
Chr17:37207165..37207193
TCACAGCAAAAAGACCAAAGC
Chr17:37207191..37207211
60.41
42.86
downstream
ENSMUSE00000142516
Chr17:37207599..37207726
GCCCTGTGGTCATTGATCTT
Chr17:37207701..37207720
59.93
50
downstream
ENSMUSE00000142509
Chr17:37208798..37208926
ATGGTGACAGGAGCGGTAAT
Chr17:37208832..37208851
59.43
50
downstream
ENSMUSE00000142514
Chr17:37209089..37209232
TTTCCAATTCACGGTTTTCC
Chr17:37209221..37209240
59.78
40
downstream
ENSMUSE00000468594
Chr17:37209798..37210377
TAAGAGGGGGATTGGAGCTT
Chr17:37210075..37210094
60.03
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
CCCCACAGCTCGTTTCTAAT
Chr17:37183757..37183777
59.19
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GTTTCCGTGACTGGGAAAACC
Chr17:37183768..37183789
62.88
52.38
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000024462