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About
UniTrap UNI34364
Trapped Gene
A330021E22Rik (
ENSMUSG00000040473
)
Vector Insertion
Chr 5: 5626008 - 5640228
Public Clones
IST13279G7 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 31% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000386172 (Chr5:5640129..5640227 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000386172 (Chr5:5640129..5640227 -)
Downstram Exon
ENSMUSE00000345486 (Chr5:5626009..5626158 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
GCTGCAGGACTTTCAGAACC
Chr5:5626002..5626021
60
55
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000347631
Chr5:5663908..5664181
AGCTCAGCAGCGTGTTTTCT
Chr5:5664093..5664112
60.34
50
upstream
ENSMUSE00000402663
Chr5:5657788..5657847
CAAGCCTATGGACCTTAATCG
Chr5:5657819..5657839
58.73
47.62
upstream
ENSMUSE00000361918
Chr5:5649790..5649855
No primer for this exon
upstream
ENSMUSE00000390808
Chr5:5646936..5647045
AAATTGAAAAGCAGCCCTGT
Chr5:5646974..5646993
58.84
40
upstream
ENSMUSE00000347856
Chr5:5644424..5644500
No primer for this exon
upstream
ENSMUSE00000386172
Chr5:5640129..5640227
No primer for this exon
upstream
ENSMUSE00000345486
Chr5:5626009..5626158
GGCACTCCTGCTTGAGAATC
Chr5:5626063..5626082
59.96
55
*** Putative Vector Insertion (Chr 5: 5626008 - 5640228) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000333667
Chr5:5625750..5625927
CTGAGGGATCTGGGTCATTG
Chr5:5625828..5625847
60.47
55
downstream
ENSMUSE00000389664
Chr5:5621920..5622043
GACTATGCCCCCTCACAAAG
Chr5:5621975..5621994
59.55
55
downstream
ENSMUSE00000402443
Chr5:5619167..5619215
AAATCCCTGGTAAAGCCACA
Chr5:5619171..5619190
59.43
45
downstream
ENSMUSE00000361655
Chr5:5618948..5619069
CTTTTTACCAAGGGGTTTTGA
Chr5:5619020..5619040
58.15
38.1
downstream
ENSMUSE00000351918
Chr5:5617169..5617385
ATGCAAGGATCCGAGAATTG
Chr5:5617171..5617190
60.04
45
downstream
ENSMUSE00000301156
Chr5:5613686..5613850
CATCTGGGCGAACTTGTTTC
Chr5:5613761..5613780
60.64
50
downstream
ENSMUSE00000301152
Chr5:5604274..5604392
CTCAGCAGGTCTGCTCATCA
Chr5:5604336..5604355
60.29
55
downstream
ENSMUSE00000301144
Chr5:5595921..5596039
CATTGTAGCCTAAACCCCTCTG
Chr5:5595930..5595951
60.01
50
downstream
ENSMUSE00000301138
Chr5:5593772..5593853
AACATCCCAAAATGCAGCAC
Chr5:5593812..5593831
60.9
45
downstream
ENSMUSE00000379846
Chr5:5589175..5589213
AATTAAAAGGCTGGCAGCAG
Chr5:5589162..5589181
59.5
45
downstream
ENSMUSE00000301133
Chr5:5589120..5589312
AACAATCTTGCCGTTCCTGT
Chr5:5589099..5589118
59.6
45
downstream
ENSMUSE00000369220
Chr5:5587141..5587278
AAGGGGCATGGTCTTTTGTT
Chr5:5587201..5587220
60.72
45
downstream
ENSMUSE00000301126
Chr5:5586356..5586432
GGCAGATAAGCCCGGTAAGT
Chr5:5586382..5586401
60.47
55
downstream
ENSMUSE00000301117
Chr5:5584651..5584848
GACGCAACCATCTTTCCAAT
Chr5:5584699..5584718
59.94
45
downstream
ENSMUSE00000301108
Chr5:5582442..5582528
CCATGACTTGTTAGCCTGCTC
Chr5:5582462..5582482
59.89
52.38
downstream
ENSMUSE00000301102
Chr5:5581904..5582008
TGTTGCATTCTGTGGTCGTT
Chr5:5581918..5581937
60.16
45
downstream
ENSMUSE00000391705
Chr5:5579282..5581366
CAGGGCAACCTAGACACCAT
Chr5:5579368..5579387
59.99
55
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TAATAATCGCCTTGCAGCAC
Chr5:5634160..5634180
58.94
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
ATCCTTCTCGCAGGTGAGTT
Chr5:5634219..5634239
58.89
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000040473