Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI34489
Trapped Gene
Lrig2 (ENSMUSG00000032913)
Vector Insertion
Chr 3: 104257906 - 104265612
Public Clones IST13899A9 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000565672 (Chr3:104265324..104265611 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCCCCAAGATGCTGCTAATC Chr3:104265423..104265442 60.18 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000565672 (Chr3:104265324..104265611 -)
Downstram Exon
ENSMUSE00000513067 (Chr3:104257907..104261887 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCCCCAAGATGCTGCTAATC Chr3:104265423..104265442 60.18 50 GAGCTACGCTGATGTGGTCA Chr3:104261539..104261558 60.02 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000360131 Chr3:104315379..104315779 TCTCTCCTCAGCCGACTCTC Chr3:104315657..104315676 59.82 60
upstream ENSMUSE00000587984 Chr3:104301412..104301477 AACTGGAACAACACCTTGGAA Chr3:104301432..104301452 59.48 42.86
upstream ENSMUSE00000587983 Chr3:104298554..104298628 No primer for this exon
upstream ENSMUSE00000587982 Chr3:104297981..104298115 TGCAGAAGCATTCGAGCTTT Chr3:104298069..104298088 61.19 45
upstream ENSMUSE00000587981 Chr3:104295612..104295755 TGATTCCACCCAAGGTCTTC Chr3:104295635..104295654 59.9 50
upstream ENSMUSE00000587980 Chr3:104295334..104295477 ATGCAGCGGAATGGAATTAG Chr3:104295382..104295401 60.06 45
upstream ENSMUSE00000587979 Chr3:104294791..104294934 GAGTGAACAAGGGGTGGCTA Chr3:104294889..104294908 60.11 55
upstream ENSMUSE00000587978 Chr3:104294453..104294596 GCCTTGATGAATCTGCCTTT Chr3:104294551..104294570 59.27 45
upstream ENSMUSE00000587977 Chr3:104285778..104285858 CTAGCGAAGCCTTTTCTGGA Chr3:104285798..104285817 59.72 50
upstream ENSMUSE00000587976 Chr3:104284032..104284103 TGCAAGGAAACCGGATTAAG Chr3:104284079..104284098 60.07 45
upstream ENSMUSE00000587975 Chr3:104283705..104283773 No primer for this exon
upstream ENSMUSE00000315278 Chr3:104272408..104272571 TCAGTGGCTGGTGGATAACA Chr3:104272501..104272520 60.11 50
upstream ENSMUSE00000315272 Chr3:104270966..104271286 TAGTGTCTTGCGCCTCTTCA Chr3:104271059..104271078 59.74 50
upstream ENSMUSE00000315265 Chr3:104269410..104269691 GATGGTGGCACTGATTTTCC Chr3:104269559..104269578 60.33 50
upstream ENSMUSE00000565673 Chr3:104267782..104268231 TCCCCCAATTGTGACTCTTC Chr3:104267937..104267956 59.9 50
upstream ENSMUSE00000488391 Chr3:104266582..104266731 GGCAGCCATCAGCAACTTAT Chr3:104266620..104266639 60.24 50
upstream ENSMUSE00000565672 Chr3:104265324..104265611 TCCCCAAGATGCTGCTAATC Chr3:104265423..104265442 60.18 50
upstream ENSMUSE00000513067 Chr3:104257907..104261887 TCCTGAGAATTGGGGAGATG Chr3:104259497..104259516 60 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGCCCAGGGTAGTCAGGGTA Chr3:104259612..104259632 60.89 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGCCCAGGGTAGTCAGGGTA Chr3:104259612..104259632 60.89 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032913