Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI3452
Trapped Gene
Nf2 (ENSMUSG00000009073)
Vector Insertion
Chr 11: 4699999 - 4703680
Public Clones AK0406 (sanger) (ggtc) CMHD-GT_352G10-3 (cmhd) CMHD-GT_320C5-3 (cmhd)
IST12640C8 (tigm) IST13638H3 (tigm) IST12553H9 (tigm) IST13639G6 (tigm)
Private Clones OST318042 (lexicon)
Severity of mutation (?) Insertion after 69% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000104225 (Chr11:4703681..4703756 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000104225 (Chr11:4703681..4703756 -)
Downstram Exon
ENSMUSE00000104215 (Chr11:4699864..4699998 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000456636 Chr11:4748875..4748988 No primer for this exon
upstream ENSMUSE00000581392 Chr11:4748875..4749368 No primer for this exon
upstream ENSMUSE00000710927 Chr11:4748875..4749530 No primer for this exon
upstream ENSMUSE00000712691 Chr11:4748875..4749530 No primer for this exon
upstream ENSMUSE00000682067 Chr11:4733001..4733136 No primer for this exon
upstream ENSMUSE00000656076 Chr11:4720371..4720496 No primer for this exon
upstream ENSMUSE00000656088 Chr11:4720371..4720496 No primer for this exon
upstream ENSMUSE00000104218 Chr11:4718508..4718630 No primer for this exon
upstream ENSMUSE00000104220 Chr11:4716085..4716168 No primer for this exon
upstream ENSMUSE00000104229 Chr11:4708260..4708328 No primer for this exon
upstream ENSMUSE00000104227 Chr11:4706257..4706339 No primer for this exon
upstream ENSMUSE00000104225 Chr11:4703681..4703756 No primer for this exon

*** Putative Vector Insertion (Chr 11: 4699999 - 4703680) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000104215 Chr11:4699864..4699998 No primer for this exon
downstream ENSMUSE00000308293 Chr11:4694846..4694920 No primer for this exon
downstream ENSMUSE00000104231 Chr11:4694415..4694528 No primer for this exon
downstream ENSMUSE00000709587 Chr11:4691094..4691216 No primer for this exon
downstream ENSMUSE00000711364 Chr11:4691094..4691216 No primer for this exon
downstream ENSMUSE00000104239 Chr11:4689668..4689885 No primer for this exon
downstream ENSMUSE00000682063 Chr11:4689668..4689885 No primer for this exon
downstream ENSMUSE00000104236 Chr11:4687447..4687552 No primer for this exon
downstream ENSMUSE00000682061 Chr11:4687447..4687552 No primer for this exon
downstream ENSMUSE00000104222 Chr11:4684439..4684566 No primer for this exon
downstream ENSMUSE00000682059 Chr11:4684439..4684566 No primer for this exon
downstream ENSMUSE00000308256 Chr11:4682190..4682355 No primer for this exon
downstream ENSMUSE00000682057 Chr11:4682190..4682355 No primer for this exon
downstream ENSMUSE00000682066 Chr11:4681940..4682355 No primer for this exon
downstream ENSMUSE00000682080 Chr11:4680590..4680625 No primer for this exon
downstream ENSMUSE00000682077 Chr11:4680581..4680625 No primer for this exon
downstream ENSMUSE00000682068 Chr11:4672337..4672641 No primer for this exon
downstream ENSMUSE00000581387 Chr11:4667668..4668276 No primer for this exon
downstream ENSMUSE00000581399 Chr11:4665851..4668276 No primer for this exon
downstream ENSMUSE00000682054 Chr11:4665851..4668276 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TCAGGACCTGGAGATGTATGG Chr11:4700701..4700722 59.94 52.38 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTCGACGTGACTGGGAAAAC Chr11:4700614..4700634 60.56 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CTGCTCTCTAATCGCCTTGC Chr11:4700694..4700714 60.26 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 AAGCCTCTGGCAGCAATAAC Chr11:4700726..4700746 59.48 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000009073