Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI34606
Trapped Gene
Tbc1d22b (ENSMUSG00000042203)
Vector Insertion
Chr 17: 29715889 - 29731621
Public Clones IST12835E3 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 65% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000234776 (Chr17:29715774..29715888 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGTCGTCTTCCTCTCGGAAT Chr17:29715863..29715882 59.8 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000234776 (Chr17:29715774..29715888 +)
Downstram Exon
ENSMUSE00000394496 (Chr17:29731622..29731728 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGTCGTCTTCCTCTCGGAAT Chr17:29715863..29715882 59.8 50 TGTATTCCGTCCAGCAGCTT Chr17:29731730..29731749 60.8 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000548944 Chr17:29686747..29686939 CAGCAAGCAGTTCTGGAAGA Chr17:29686898..29686917 59.31 50
upstream ENSMUSE00000417039 Chr17:29701377..29701433 GGATCCTCGGCTCACAAAAA Chr17:29701413..29701432 62.41 50
upstream ENSMUSE00000417031 Chr17:29705337..29705644 ACTGCAGCCCAAGTCCTAGA Chr17:29705509..29705528 60.01 55
upstream ENSMUSE00000417027 Chr17:29707532..29707711 AATGACAGTCCGGGAGAAAA Chr17:29707641..29707660 59.53 45
upstream ENSMUSE00000417022 Chr17:29708610..29708680 CAGTGACCTGGAGACTGCTG Chr17:29708658..29708677 59.6 60
upstream ENSMUSE00000234820 Chr17:29709915..29710043 AATGAAGAGCACCACCAGGA Chr17:29710011..29710030 60.66 50
upstream ENSMUSE00000393319 Chr17:29712101..29712166 GAACCCTCTCATCCCCTTGT Chr17:29712124..29712143 60.31 55
upstream ENSMUSE00000234776 Chr17:29715774..29715888 TGTCGTCTTCCTCTCGGAAT Chr17:29715863..29715882 59.8 50

*** Putative Vector Insertion (Chr 17: 29715889 - 29731621) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000394496 Chr17:29731622..29731728 TGTATTCCGTCCAGCAGCTT Chr17:29731730..29731749 60.8 50
downstream ENSMUSE00000234625 Chr17:29732793..29732868 TGGCTGTGCAAACGTGTAAT Chr17:29732819..29732838 60.18 45
downstream ENSMUSE00000234607 Chr17:29736784..29736911 CTGCAGGTACTCCACCTCGT Chr17:29736833..29736852 60.32 60
downstream ENSMUSE00000234586 Chr17:29737091..29737186 GAGATGGAAGTGCGAGAAGC Chr17:29737126..29737145 60.1 55
downstream ENSMUSE00000472519 Chr17:29741789..29743751 TGCTTCACCCTGGAAATAGG Chr17:29742977..29742996 60.07 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACTCACAGCCGGGTACATTC Chr17:29718873..29718893 60 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACTCACAGCCGGGTACATTC Chr17:29718873..29718893 60 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000042203