Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI34828
Trapped Gene
Aurkc (ENSMUSG00000070837)
Vector Insertion
Chr 7: 6952839 - 6955067
Public Clones IST13758F4 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 66% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000600444 (Chr7:6952690..6952838 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AGACTTTGGGTGGTCTGTGC Chr7:6952803..6952822 60.16 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000600444 (Chr7:6952690..6952838 +)
Downstram Exon
ENSMUSE00000537783 (Chr7:6955068..6955242 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AGACTTTGGGTGGTCTGTGC Chr7:6952803..6952822 60.16 55 CGGTTTCTGCGCTATCATTT Chr7:6955131..6955150 60.23 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000600456 Chr7:6948154..6948259 GCTGGGATCTACGAGACTGC Chr7:6948164..6948183 59.98 60
upstream ENSMUSE00000677184 Chr7:6948979..6949012 No primer for this exon
upstream ENSMUSE00000600447 Chr7:6949230..6949421 AAGTGGAGATCCAGGCACAC Chr7:6949397..6949416 60.12 55
upstream ENSMUSE00000600452 Chr7:6950225..6950363 GAAGTTGGACCAGCAGCGTA Chr7:6950336..6950355 61.38 55
upstream ENSMUSE00000600444 Chr7:6952690..6952838 AGACTTTGGGTGGTCTGTGC Chr7:6952803..6952822 60.16 55

*** Putative Vector Insertion (Chr 7: 6952839 - 6955067) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000537783 Chr7:6955068..6955242 CGGTTTCTGCGCTATCATTT Chr7:6955131..6955150 60.23 45
downstream ENSMUSE00000537808 Chr7:6955504..6955799 CCTTCGAGAGTGTTCCCTGA Chr7:6955644..6955663 60.38 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGACTTTGGGTGGTCTGTGC Chr7:6952804..6952824 60.16 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGACTTTGGGTGGTCTGTGC Chr7:6952804..6952824 60.16 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000070837