Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI3494
Trapped Gene
Bcl2l1 (ENSMUSG00000007659)
Vector Insertion
Chr 2: 152655035 - 152655162
Public Clones AS0758 (sanger) AS0677 (sanger) AL0418 (sanger) AQ0716 (sanger)
AR0408 (sanger) AN0071 (sanger) AV0131 (sanger) AQ1036 (sanger)
AR1208 (sanger) AV0627 (sanger) AR0493 (sanger) AS0525 (sanger)
AQ0670 (sanger) AF0495 (sanger) AQ0669 (sanger) AP0696 (sanger)
AS0993 (sanger) AR1189 (sanger) AR0269 (sanger) AS0680 (sanger)
AT0199 (sanger) AR0256 (sanger) AR1180 (sanger) AQ0654 (sanger)
AV0189 (sanger) AQ0432 (sanger) AL0662 (sanger) AQ0666 (sanger)
AR1002 (sanger) H002E05 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 80% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000381992 (Chr2:152655163..152655838 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000381992 (Chr2:152655163..152655838 -)
Downstram Exon
ENSMUSE00000681711 (Chr2:152655023..152655034 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000556013 Chr2:152657339..152657464 No primer for this exon
upstream ENSMUSE00000681713 Chr2:152656122..152656402 No primer for this exon
upstream ENSMUSE00000381992 Chr2:152655163..152655838 No primer for this exon
upstream ENSMUSE00000713045 Chr2:152655163..152655838 No primer for this exon

*** Putative Vector Insertion (Chr 2: 152655035 - 152655162) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000681711 Chr2:152655023..152655034 No primer for this exon
downstream ENSMUSE00000556001 Chr2:152606408..152608018 No primer for this exon
downstream ENSMUSE00000595156 Chr2:152606404..152608018 No primer for this exon
downstream ENSMUSE00000556011 Chr2:152593052..152593195 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTAAGAACCACGCCCCTTGT Chr2:152655141..152655161 61.29 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTCTCGTGACTGGGAAAACC Chr2:152655095..152655115 60.09 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000007659