Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI35424
Trapped Gene
AC084054.12-202 (ENSMUSG00000079504)
Vector Insertion
Chr 5: 53923588 - 53923899
Public Clones IST10973F7 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 19% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000697269 (Chr5:53923581..53923587 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000697269 (Chr5:53923581..53923587 +)
Downstram Exon
ENSMUSE00000697268 (Chr5:53923900..53923928 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000697269 Chr5:53923581..53923587 No primer for this exon

*** Putative Vector Insertion (Chr 5: 53923588 - 53923899) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000697268 Chr5:53923900..53923928 No primer for this exon
downstream ENSMUSE00000697266 Chr5:53926112..53926120 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000079504