Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI3556
Trapped Gene
Gtpbp4 (ENSMUSG00000021149)
Vector Insertion
Chr 13: 8992062 - 8995194
Public Clones BA0470 (sanger) D019A08 (ggtc) PST20210-NR (escells)
Private Clones OST369593 (lexicon) OST194719 (lexicon)
Severity of mutation (?) Insertion after 3% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000477746 (Chr13:8995195..8995263 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000477746 (Chr13:8995195..8995263 -)
Downstram Exon
ENSMUSE00000115107 (Chr13:8991891..8992061 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000477746 Chr13:8995195..8995263 No primer for this exon

*** Putative Vector Insertion (Chr 13: 8992062 - 8995194) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000115107 Chr13:8991891..8992061 No primer for this exon
downstream ENSMUSE00000115082 Chr13:8991178..8991281 No primer for this exon
downstream ENSMUSE00000115106 Chr13:8990961..8991097 No primer for this exon
downstream ENSMUSE00000115095 Chr13:8989944..8990044 No primer for this exon
downstream ENSMUSE00000115084 Chr13:8988299..8988391 No primer for this exon
downstream ENSMUSE00000115089 Chr13:8987002..8987193 No primer for this exon
downstream ENSMUSE00000115087 Chr13:8986495..8986560 No primer for this exon
downstream ENSMUSE00000115097 Chr13:8984884..8984973 No primer for this exon
downstream ENSMUSE00000252316 Chr13:8984455..8984565 No primer for this exon
downstream ENSMUSE00000252305 Chr13:8979148..8979225 No primer for this exon
downstream ENSMUSE00000252297 Chr13:8978675..8978726 No primer for this exon
downstream ENSMUSE00000115101 Chr13:8977774..8977874 No primer for this exon
downstream ENSMUSE00000462027 Chr13:8976482..8976679 No primer for this exon
downstream ENSMUSE00000115086 Chr13:8974191..8974256 No primer for this exon
downstream ENSMUSE00000115104 Chr13:8973410..8973553 No primer for this exon
downstream ENSMUSE00000454482 Chr13:8971317..8972520 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTATCTCCATTTCCCCGACA Chr13:8995147..8995167 59.89 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTATCTCCATTTCCCCGACA Chr13:8995147..8995167 59.89 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 GCATTCTTGAGGAGGGTTGA Chr13:8995262..8995282 60.19 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 GCATTCTTGAGGAGGGTTGA Chr13:8995262..8995282 60.19 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000021149