Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI35800
Trapped Gene
Hist1h2bp (ENSMUSG00000069308)
Vector Insertion
Chr 13: 21879846 - 21880892
Public Clones IST13055G1 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000660932 (Chr13:21879377..21879845 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCGTGAACGACATCTTCGAG Chr13:21879580..21879599 59.98 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000660932 (Chr13:21879377..21879845 +)
Downstram Exon
ENSMUSE00000571614 (Chr13:21880893..21880952 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCGTGAACGACATCTTCGAG Chr13:21879580..21879599 59.98 50 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000684052 Chr13:21879357..21879760 TCGTGAACGACATCTTCGAG Chr13:21879580..21879599 59.98 50
upstream ENSMUSE00000660932 Chr13:21879377..21879845 TCGTGAACGACATCTTCGAG Chr13:21879580..21879599 59.98 50
upstream ENSMUSE00000571615 Chr13:21879384..21879668 TCGTGAACGACATCTTCGAG Chr13:21879580..21879599 59.98 50
upstream ENSMUSE00000643298 Chr13:21879671..21879760 GGCTGTCACCAAGTACACCA Chr13:21879734..21879753 59.6 55

*** Putative Vector Insertion (Chr 13: 21879846 - 21880892) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000571614 Chr13:21880893..21880952 No primer for this exon
downstream ENSMUSE00000684051 Chr13:21880893..21881079 CACACTTCGGTCTCCACTCA Chr13:21881010..21881029 59.86 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCCACCCACATTCTCAAAAC Chr13:21879806..21879826 60.36 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCCACCCACATTCTCAAAAC Chr13:21879806..21879826 60.36 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000069308