Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI35834
Trapped Gene
OTTMUSG00000016325 (ENSMUSG00000078863)
Vector Insertion
Chr 2: 177564275 - 177567802
Public Clones IST14111G12 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000678565 (Chr2:177567362..177567801 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GAACATGGAATGTCCTGCAA Chr2:177567636..177567655 59.5 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000678565 (Chr2:177567362..177567801 -)
Downstram Exon
ENSMUSE00000678562 (Chr2:177564276..177567801 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GAACATGGAATGTCCTGCAA Chr2:177567636..177567655 59.5 45 CATCTTCTGCACGGTTCTCA Chr2:177564954..177564973 59.98 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000678563 Chr2:177574942..177575023 TGTGATGTGTTCTGCGTGAC Chr2:177574993..177575012 59.27 50
upstream ENSMUSE00000678568 Chr2:177574942..177575004 TGCGTGACTCTACTGGGTGT Chr2:177574981..177575000 59.34 55
upstream ENSMUSE00000678567 Chr2:177569225..177569351 GGCTTTGCTGGATCCTTCTC Chr2:177569282..177569301 61.24 55
upstream ENSMUSE00000678566 Chr2:177568956..177569016 No primer for this exon
upstream ENSMUSE00000678565 Chr2:177567362..177567801 GAACATGGAATGTCCTGCAA Chr2:177567636..177567655 59.5 45
upstream ENSMUSE00000678561 Chr2:177566496..177567801 AAAGCCTTCGCAAGCAGTAG Chr2:177566638..177566657 59.79 50
upstream ENSMUSE00000678564 Chr2:177566496..177566857 AAAGCCTTCGCAAGCAGTAG Chr2:177566638..177566657 59.79 50
upstream ENSMUSE00000678562 Chr2:177564276..177567801 TGCACAAAGCAGTCATCTCC Chr2:177566546..177566565 59.99 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTTCTGCAGAGCAACCCTCT Chr2:177567766..177567786 59.6 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCAATGTCGTGACTGGGAAA Chr2:177567738..177567758 60.09 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000078863