Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI36142
Trapped Gene
Vangl1 (ENSMUSG00000027860)
Vector Insertion
Chr 3: 101962118 - 101967457
Public Clones CMHD-GT_460D4-3 (cmhd) IST15076C6 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 84% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000173682 (Chr3:101967222..101967456 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CAGCAGCACTACCACAGCAT Chr3:101967277..101967296 60.08 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000173682 (Chr3:101967222..101967456 -)
Downstram Exon
ENSMUSE00000396395 (Chr3:101962119..101962420 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CAGCAGCACTACCACAGCAT Chr3:101967277..101967296 60.08 55 GAGCCATCGATCCTTGTCAT Chr3:101962336..101962355 60.04 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000453892 Chr3:102008435..102008531 No primer for this exon
upstream ENSMUSE00000453884 Chr3:102000715..102000899 ATCAGACGAAGCTTGCCATT Chr3:102000789..102000808 59.84 45
upstream ENSMUSE00000453879 Chr3:101993332..101993470 AGATGGCAGAGGGTCAGAAA Chr3:101993412..101993431 59.8 50
upstream ENSMUSE00000453875 Chr3:101987874..101988481 CATCGTGCAATACGCAGTCT Chr3:101988013..101988032 59.9 50
upstream ENSMUSE00000370676 Chr3:101970761..101970894 CGAACCTCCTAACAGCATCC Chr3:101970813..101970832 59.69 55
upstream ENSMUSE00000173686 Chr3:101969278..101969410 ATAACGCCACTGGTCAGTCC Chr3:101969382..101969401 60 55
upstream ENSMUSE00000173682 Chr3:101967222..101967456 CAGCAGCACTACCACAGCAT Chr3:101967277..101967296 60.08 55
upstream ENSMUSE00000396395 Chr3:101962119..101962420 GGCTGTGACAAACGGGTTAC Chr3:101962309..101962328 60.42 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCATATCCAGCGTCTCCAGT Chr3:101964405..101964425 60.1 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCCCCTTGTGCAGAGGAATA Chr3:101964420..101964440 60.59 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CTTCTGCATCACCAACAGCA Chr3:101964231..101964251 61.02 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 CTTCTGCATCACCAACAGCA Chr3:101964231..101964251 61.02 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000027860