Tools for the biologist enabling optimized use of gene trap clones
Homepage
|
Blast Search
|
GO Search
|
Advanced Search
|
About
UniTrap UNI36195
Trapped Gene
Efhb (
ENSMUSG00000023931
)
Vector Insertion
Chr 17: 53591885 - 53602647
Public Clones
IST12901H12 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 33% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000370133 (Chr17:53601756..53602646 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CACCTGTGTCGTGATGAACC
Chr17:53601998..53602017
60.01
55
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000370133 (Chr17:53601756..53602646 -)
Downstram Exon
ENSMUSE00000136456 (Chr17:53591886..53591948 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CACCTGTGTCGTGATGAACC
Chr17:53601998..53602017
60.01
55
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000370133
Chr17:53601756..53602646
CACCTGTGTCGTGATGAACC
Chr17:53601998..53602017
60.01
55
upstream
ENSMUSE00000136456
Chr17:53591886..53591948
TGCAACCTGTTTGACTGAGAA
Chr17:53591896..53591916
59.47
42.86
*** Putative Vector Insertion (Chr 17: 53591885 - 53602647) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000136458
Chr17:53590876..53591019
ATTTGGGGGTCATTTGCTCT
Chr17:53590900..53590919
60.69
45
downstream
ENSMUSE00000136452
Chr17:53588710..53588890
GAAACGTGGTAATGGGCTGT
Chr17:53588829..53588848
59.86
50
downstream
ENSMUSE00000136447
Chr17:53585070..53585180
TTCGAAGGATTTCGATGGAT
Chr17:53585115..53585134
59.46
40
downstream
ENSMUSE00000136449
Chr17:53576409..53576538
TGCCGGGTTATATTTTCGAT
Chr17:53576485..53576504
59.28
40
downstream
ENSMUSE00000136445
Chr17:53566235..53566318
No primer for this exon
downstream
ENSMUSE00000136457
Chr17:53565537..53565604
GACCTGGGGGAACATTCATA
Chr17:53565551..53565570
59.6
50
downstream
ENSMUSE00000136455
Chr17:53561200..53561354
GAAAGCCACCTGCAAAGTGT
Chr17:53561193..53561212
60.3
50
downstream
ENSMUSE00000136454
Chr17:53552788..53552995
TCCTGGTCCACGTCACAGTA
Chr17:53552858..53552877
60.15
55
downstream
ENSMUSE00000136459
Chr17:53540760..53540972
CCACAGCGTTGATCTCAGAA
Chr17:53540759..53540778
59.98
50
downstream
ENSMUSE00000321516
Chr17:53540071..53540252
TGTCAGATCGAATGGTTGGA
Chr17:53540192..53540211
60.05
45
downstream
ENSMUSE00000395326
Chr17:53538214..53538444
CATGGCCGTTTTACACTTGA
Chr17:53538252..53538271
59.58
45
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
CCAGTGTCCTAGGCAGGAAG
Chr17:53593627..53593647
59.86
60
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TGTGTTCGTGACTGGGAAAA
Chr17:53593582..53593602
60.13
45
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000023931