Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI36298
Trapped Gene
Dvl3 (ENSMUSG00000003233)
Vector Insertion
Chr 16: 20517429 - 20522328
Public Clones IST14972B11 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 7% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000414406 (Chr16:20517137..20517428 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCGGCCCAGCTATAAGTTCT Chr16:20517381..20517400 60.73 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000414406 (Chr16:20517137..20517428 +)
Downstram Exon
ENSMUSE00000561563 (Chr16:20522329..20522398 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCGGCCCAGCTATAAGTTCT Chr16:20517381..20517400 60.73 55 CAGGGTAGCTTGGCATTGTC Chr16:20522376..20522395 60.66 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000414406 Chr16:20517137..20517428 GCGGCCCAGCTATAAGTTCT Chr16:20517381..20517400 60.73 55

*** Putative Vector Insertion (Chr 16: 20517429 - 20522328) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000561563 Chr16:20522329..20522398 CAGGGTAGCTTGGCATTGTC Chr16:20522376..20522395 60.66 55
downstream ENSMUSE00000130957 Chr16:20523483..20523604 GGGTTGTCAGCACAGAAAGG Chr16:20523541..20523560 60.69 55
downstream ENSMUSE00000130953 Chr16:20523725..20523834 GAGTCGGTCTCCGTGTCATT Chr16:20523784..20523803 60.12 55
downstream ENSMUSE00000130947 Chr16:20524013..20524148 GTAGTCTCCAGCTCGCTGCT Chr16:20524112..20524131 59.92 60
downstream ENSMUSE00000130945 Chr16:20524325..20524418 TGCTCTGTTCTGTGGAGCTG Chr16:20524353..20524372 60.33 55
downstream ENSMUSE00000130938 Chr16:20524592..20524661 GTGATGCTGCTGAAAGACGA Chr16:20524617..20524636 60.14 50
downstream ENSMUSE00000561557 Chr16:20525712..20525851 ATCACCTCGCTCATTGCTTT Chr16:20525770..20525789 59.84 45
downstream ENSMUSE00000561555 Chr16:20525979..20526055 CACAATCTCCCGAAGGACTC Chr16:20526047..20526066 59.65 55
downstream ENSMUSE00000130939 Chr16:20526162..20526229 GTCCCAGCACTTGGCTACAG Chr16:20526198..20526217 60.85 60
downstream ENSMUSE00000130936 Chr16:20526340..20526489 GTGCTTAAGGAGGGGCTCAT Chr16:20526442..20526461 60.6 55
downstream ENSMUSE00000130962 Chr16:20527022..20527153 GATGAAAGCATTGGGAATGG Chr16:20527155..20527174 60.27 45
downstream ENSMUSE00000130933 Chr16:20527325..20527492 ACAGAGGTCGCCAAAGATGT Chr16:20527491..20527510 59.73 50
downstream ENSMUSE00000130960 Chr16:20530850..20531065 TCATGGTCGTGAAGCGATAG Chr16:20530883..20530902 59.82 50
downstream ENSMUSE00000315994 Chr16:20531153..20531589 ACTTGGAGTCCCCAGCTTTT Chr16:20531233..20531252 60.11 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTAATCGCCTTGCAGCACAT Chr16:20520479..20520499 61.32 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CGGCCCAGCTATAAGTTCTTC Chr16:20517383..20517404 60.23 52.38 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000003233